Totten Glacier ocean & sediment DNA (IN2017_V01)

Maintenance and Update Frequency: notPlanned Statement: We extracted and analysed modern (water column) and ancient (sediments) marine bacterial and eukaryotic DNA of the Totten Glacier region. A combination of 16S and 18S rRNA amplicon sequencing (modern DNA) and shotgun metagenomics (sedimentary a...

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Bibliographic Details
Other Authors: AODN Data Manager (distributor), Ambrecht, Linda (hasPrincipalInvestigator), Armbrecht, Linda (hasPrincipalInvestigator), Data Officer (distributor), Focardi, Amaranta (owner), IMAS Data Manager (hasAssociationWith), Institute for Marine and Antarctic Studies (IMAS) (resourceProvider), Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) (hasAssociationWith), University of Technology Sydney (hasAssociationWith), Werkman, Harko (custodian)
Format: Dataset
Language:unknown
Published: Australian Ocean Data Network
Subjects:
Online Access:https://researchdata.edu.au/totten-glacier-ocean-dna-in2017v01/2305605
Description
Summary:Maintenance and Update Frequency: notPlanned Statement: We extracted and analysed modern (water column) and ancient (sediments) marine bacterial and eukaryotic DNA of the Totten Glacier region. A combination of 16S and 18S rRNA amplicon sequencing (modern DNA) and shotgun metagenomics (sedimentary ancient DNA, sedaDNA) was used. We explore environmental and geochemical variables driving biodiversity patterns of bacteria and eukaryotes. This record presents genetic data underlying the paper 'From the Surface Ocean to the Seafloor: Linking Modern and Paleo-genetics at the Sabrina Coast, East Antarctica (IN2017_V01)' by Armbrecht et al. In this study, we provide the first taxonomic overview of the modern and ancient marine bacterial and eukaryotic communities of the Totten Glacier region, East Antarctica, using a combination of 16S and 18S rRNA amplicon sequencing (modern DNA) and shotgun metagenomic (sedimentary ancient DNA, sedaDNA) analyses, respectively. We explore environmental and geochemical variables that drive these biodiversity patterns. Our data show considerable differences between eukaryote and bacterial signals detected via DNA analyses in the water column vs. the sediments. Organisms that are well represented in deeper waters appear are to have a higher likelihood of becoming preserved in the sediments. The study provides the first assessment of DNA transfer from ocean waters to sediments, while also providing a broad overview of the biological communities occurring in the climatically important Totten Glacier region. (Please note that this record is mirrored in the UTAS Research Data Portal, here: https://rdp.utas.edu.au/metadata/8628529b-49cf-42d4-9459-3c1e97f70d98)