| Summary: | Maintenance and Update Frequency: asNeeded Statement: Established methods for sponge collection were used see: Botté ES, Nielsen S, Abdul Wahab MA, Webster J, Robbins S, Thomas T, et al. Changes in the metabolic potential of the sponge microbiome under ocean acidification. Nat Commun. 2019;10:1–10.\n Sponge species were identified based on their morphological characters by taxonomic specialists at the Western Australian Museum (Perth, Australia).\n Samples were prepared and extracted following the Earth Microbiome Project (EMP) protocol Nothias LF. Earth Microbiome Project (EMP) protocol for the LC-MS/MS. Available from: https://github.com/biocore/emp/blob/master/protocols/MetabolomicsLC.md\n Results of the DEREPLICATOR workflow can be accessed at https://gnps.ucsd.edu/ProteoSAFe/status.jsp?task=31be4f1f9d2e46a99823811ddd0cfd70. \n Schymanski EL, Jeon J, Gulde R, Fenner K, Ruff M, Singer HP, et al. Identifying small molecules via high resolution mass spectrometry: communicating confidence. Environ Sci Technol. 2014;48:2097–8 \n QC files are available from MassIVE Repository. See Zhang et al. 2022 for futher details. \n Credit Couvillion, SP. Pacific Northwest Laboratory, Richland, WA, USA Credit Zhang, S. University of New South Wales (UNSW) Credit Song, W. (UNSW) Credit Webster, N. Australian Institute of Marine Science (AIMS) and University of Queensland (UQ) Credit Thomas, T. University of New South Wales (UNSW) Credit Nothias, LF. University of California San Diego, USA Four species of marine sponges were collected from Davies Reef at 4 - 9m depths. Four biological replicates of each of Carteriospongia foliascens, Cliona orientalis, Coscinoderma matthewsi, Ircinia ramosa, Pericharax heteroaphis, and Stylissa flabelliformis were placed into separated plastic bags filled with seawater and brought to the surface. Following collection, part of the tissue from each specimen was immediately preserved in 70% ethanol for species classification and the remaining tissue was snap-frozen in liquid ...
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