Moulting and body shrinkage in the Antarctic krill, Euphausia superba
Euphausia superba were collected from the Antarctic Ocean during the cruise by RV Kaiyo-Maru in January 1980, and were transported to the Australian Institute of Marine Science laboratories at Townsville in February 1980.\n\nSpecimens of Euphausia superba were maintained in a cold room at -0.5°C (0...
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| Format: | Dataset |
| Language: | unknown |
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data.gov.au
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| Online Access: | https://researchdata.edu.au/moulting-body-shrinkage-euphausia-superba/1924410 http://data.gov.au/dataset/3150610a-303b-43b0-ad66-a4acaecaf0ee |
| Summary: | Euphausia superba were collected from the Antarctic Ocean during the cruise by RV Kaiyo-Maru in January 1980, and were transported to the Australian Institute of Marine Science laboratories at Townsville in February 1980.\n\nSpecimens of Euphausia superba were maintained in a cold room at -0.5°C (0 to -1.0°C) under continuous subdued light (< 0.6 W/m²) for a period of 5 months. Seawater was collected from the adjacent Coral Sea and the salinity adjusted to 34.0 ppt with distilled water. Single specimens were isolated into 1-4 litre glass beakers, depending on size. Approximately 80% of seawater in the beakers was changed once a week, when new food was prepared. One of three feeds was provided to each group of 15 specimens of various sizes: a mixture of laboratory cultures of microalgae (Dunaliella tertiolecta and Phaeodactylum tricornutum); an artificial pet fish food (Tetra Marin, W. Germany); and filtered seawater (HA Millipore filter, 0.45µm pore size) without food. When a specimen died, it was replaced with another of similar size from stock specimens, which were maintained on mixed foods (mixed microalgae and Tetra Marin). Care was taken to ensure that food was always present during this experiment.\n\nSpecimens were checked daily for moults, which were then preserved with a few drops of buffered formalin (40%) in seawater. The exopodite length of the moult uropod was later measured. Changes in body wet weight or length were then tracked by applying allometric equations derived from specimens sacrificed at the end of experiments. Preserved moults were later rinsed in distilled water to remove formalin and salts, and were dried in a desiccator over silica gel at room temperature to obtain dry weights. Fresh moults, collected from stock specimens were used for analysis of carbon and nitrogen using a elemental analyser (Perkin Elmer, model 240) with acetanilide as standard.\n\nIn another experiment, conducted over a period of 211 days, using the same temperature, light and seawater conditions described ... |
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