Environmental DNA metabarcoding for monitoring metazoan biodiversity in Antarctic nearshore ecosystems

• Other Authors: CLARKE, LAURENCE (hasPrincipalInvestigator), CLARKE, LAURENCE (processor), SUTER, LEONIE (hasPrincipalInvestigator), SUTER, LEONIE (processor), POLANOWSKI, ANDREA (hasPrincipalInvestigator), DEAGLE, BRUCE (hasPrincipalInvestigator), STARK, JONNY (hasPrincipalInvestigator), JOHNSTONE, GLENN JAMES (hasPrincipalInvestigator), JOHNSTONE, GLENN JAMES (processor), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: biota; oceans; MOLLUSKS; EARTH SCIENCE; BIOLOGICAL CLASSIFICATION; ANIMALS/INVERTEBRATES; ROTIFERS; SEGMENTED WORMS (ANNELIDS); ARTHROPODS; ECHINODERMS; CNIDARIANS; EARTH SCIENCE > BIOSPHERE > ECOSYSTEMS > MARINE ECOSYSTEMS > BENTHIC; ENVIRONMENTAL DNA; DNA METABARCODING; CYTOCHROME C OXIDASE SUBUNIT I (COI); METAZOAN; NEMATODES; CNIDARIA; CHORDATES; ANNELIDS; MOLLUSCS; ILLUMINA MISEQ; ADS > Automated DNA Sequencer; LABORATORY; GEOGRAPHIC REGION > POLAR; CONTINENT > ANTARCTICA > VESTFOLD HILLS; Abatus Bay; Antarctic; East Antarctica; Ellis Fjord; Powell Point; Shirokaya Bay; Vestfold; Vestfold Hills; Weddell; Weddell Arm; Antarc*; Antarctica
• Online Access: https://researchdata.edu.au/environmental-dna-metabarcoding-nearshore-ecosystems/1822005; https://doi.org/10.26179/caaz-b854; https://data.aad.gov.au/metadata/records/AAS_5097_DAP_nearshore_benthic_DNA_metabarcoding; http://nla.gov.au/nla.party-617536

Our aim was to compare water and sediment as sources of environmental DNA (eDNA) to better characterise Antarctic benthic communities and further develop practical approaches for DNA-based biodiversity assessment in remote environments. We used a cytochrome c oxidase subunit I (COI) metabarcoding approach to characterise metazoan communities in 26 nearshore sites across 12 locations (including Ellis Fjord, Warriner Channel, Hawker Channel, Abatus Bay, Powell Point, Shirokaya Bay, and Weddell Arm) in the Vestfold Hills (East Antarctica) based on DNA extracted from either sediment cores or filtered seawater. We detected a total of 99 metazoan species from 12 phyla (including nematodes, cnidaria, echinoderms, chordates, arthropods, annelids, rotifers and molluscs) across 26 sites, with similar numbers of species detected in sediment and water eDNA samples. Please cite: Clarke LJ et al. (2021). Environmental DNA metabarcoding for monitoring metazoan biodiversity in Antarctic nearshore ecosystems. PeerJ, DOI:10.7717/peerj.12458 This work was completed as part of the Davis Aerodrome Project (DAP).