Environmental DNA metabarcoding for monitoring metazoan biodiversity in Antarctic nearshore ecosystems

Our aim was to compare water and sediment as sources of environmental DNA (eDNA) to better characterise Antarctic benthic communities and further develop practical approaches for DNA-based biodiversity assessment in remote environments. We used a cytochrome c oxidase subunit I (COI) metabarcoding ap...

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Bibliographic Details
Other Authors: CLARKE, LAURENCE (hasPrincipalInvestigator), CLARKE, LAURENCE (processor), SUTER, LEONIE (hasPrincipalInvestigator), SUTER, LEONIE (processor), POLANOWSKI, ANDREA (hasPrincipalInvestigator), DEAGLE, BRUCE (hasPrincipalInvestigator), STARK, JONNY (hasPrincipalInvestigator), JOHNSTONE, GLENN JAMES (hasPrincipalInvestigator), JOHNSTONE, GLENN JAMES (processor), Australian Antarctic Data Centre (publisher)
Format: Dataset
Language:unknown
Published: Australian Antarctic Data Centre
Subjects:
biota
oceans
MOLLUSKS
EARTH SCIENCE
BIOLOGICAL CLASSIFICATION
ANIMALS/INVERTEBRATES
ROTIFERS
SEGMENTED WORMS (ANNELIDS)
ARTHROPODS
ECHINODERMS
CNIDARIANS
EARTH SCIENCE &gt
BIOSPHERE &gt
ECOSYSTEMS &gt
MARINE ECOSYSTEMS &gt
BENTHIC
ENVIRONMENTAL DNA
DNA METABARCODING
CYTOCHROME C OXIDASE SUBUNIT I (COI)
METAZOAN
NEMATODES
CNIDARIA
CHORDATES
ANNELIDS
MOLLUSCS
ILLUMINA MISEQ
ADS &gt
Automated DNA Sequencer
LABORATORY
GEOGRAPHIC REGION &gt
POLAR
CONTINENT &gt
ANTARCTICA &gt
VESTFOLD HILLS
Abatus Bay
Antarctic
East Antarctica
Ellis Fjord
Powell Point
Shirokaya Bay
Vestfold
Vestfold Hills
Weddell
Weddell Arm
Antarc*
Antarctica
Online Access:https://researchdata.edu.au/environmental-dna-metabarcoding-nearshore-ecosystems/1822005
https://doi.org/10.26179/caaz-b854
https://data.aad.gov.au/metadata/records/AAS_5097_DAP_nearshore_benthic_DNA_metabarcoding
http://nla.gov.au/nla.party-617536
Description
Summary:Our aim was to compare water and sediment as sources of environmental DNA (eDNA) to better characterise Antarctic benthic communities and further develop practical approaches for DNA-based biodiversity assessment in remote environments. We used a cytochrome c oxidase subunit I (COI) metabarcoding approach to characterise metazoan communities in 26 nearshore sites across 12 locations (including Ellis Fjord, Warriner Channel, Hawker Channel, Abatus Bay, Powell Point, Shirokaya Bay, and Weddell Arm) in the Vestfold Hills (East Antarctica) based on DNA extracted from either sediment cores or filtered seawater. We detected a total of 99 metazoan species from 12 phyla (including nematodes, cnidaria, echinoderms, chordates, arthropods, annelids, rotifers and molluscs) across 26 sites, with similar numbers of species detected in sediment and water eDNA samples. Please cite: Clarke LJ et al. (2021). Environmental DNA metabarcoding for monitoring metazoan biodiversity in Antarctic nearshore ecosystems. PeerJ, DOI:10.7717/peerj.12458 This work was completed as part of the Davis Aerodrome Project (DAP).

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