Toxicity of diesel contaminated soil to an Antarctic nematode worm throughout a laboratory-based ageing experiment

• Other Authors: BROWN, KATHRYN EUNICE (hasPrincipalInvestigator), BROWN, KATHRYN EUNICE (processor), KING, CATHERINE K. (hasPrincipalInvestigator), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: biota; environment; ROUNDWORMS; EARTH SCIENCE; BIOLOGICAL CLASSIFICATION; ANIMALS/INVERTEBRATES; CONTAMINANTS; TERRESTRIAL HYDROSPHERE; WATER QUALITY/WATER CHEMISTRY; EARTH SCIENCE > TERRESTRIAL HYDROSPHERE > WATER QUALITY/WATER CHEMISTRY > CONTAMINANTS > PETROLEUM HYDROCARBONS; TOXICITY LEVELS; BIOSPHERE; ECOLOGICAL DYNAMICS; ECOTOXICOLOGY; MICROFAUNA; LAND SURFACE; SOILS; SOIL CHEMISTRY; NEMATODES; DIESEL; LABORATORY OBSERVATION; LABORATORY CHEMICAL ANALYSIS; MICROSCOPES; GC-FID > Gas Chromatography - Flame Ionization Detector; LABORATORY; GEOGRAPHIC REGION > POLAR; CONTINENT > ANTARCTICA > CASEY; AUSTRALIA/NEW ZEALAND > AUSTRALIA > KINGSTON; Antarctic; Fid; New Zealand; The Antarctic; Antarc*; Antarctica
• Online Access: https://researchdata.edu.au/toxicity-diesel-contaminated-ageing-experiment/1821996; https://data.aad.gov.au/metadata/records/AAS_4100_FuelToxicityTerrestrial_AntarcticNematode-FuelAgeingExpt-KBrown; http://nla.gov.au/nla.party-617536

Clean field-collected Antarctic soil was spiked with diesel fuel to a concentration of 40,000 mg/kg soil dry weight and distributed as 110 g subsamples into microcosms (125 mL glass jars). Microscoms were kept in a temperature controlled cabinet at 3°C for up to 45 weeks. This temperature is field-realistic for Antarctic soils during summer. Throughout the 45 week ageing period, microcosms were sampled periodically (at 0, 0.3, 2, 6, 9, 12, 21, 27, 33 and 45 weeks) and used to prepare elutriates for toxicity testing. Preparation of elutriates included mixing soil with ultrapure water (MilliQ) in a 1:1 v/v ratio then centrifuging to separate the aqueous portion (elutriate) to allow it to be collected and filtered to 0.45 μm. A control elutriate was prepared in the same manner using unspiked soil. A dilution series was prepared from each batch of elutriate at: 0, 25, 50, 75 and 100% elutriate for use in toxicity tests with the nematode worm Plectus murrayi, from cultures bred at the Antarctic Division laboratory in Kingston (Tasmania, Australia). Toxicity tests followed protocols developed for this Antarctic species as described by Brown et al. (2020). Elutriate subsamples (undiluted) were analysed for a comprehensive suite of hydrocarbon chemical analytes, with and without silica gel clean up to allow measurement of polar vs non-polar compounds.