Krill Ocean Acidification Physiology Data

• Other Authors: Aker Biomarine (hasAssociationWith), Australian Antarctic Division (AAD), Department of the Environment (DoE), Australian Government (hasAssociationWith), CSIRO Oceans & Atmosphere Flagship (hasAssociationWith), Ericson, Jessica (owner), Hellessey, Nicole (owner), Hoem, Nils (owner), IMAS Data Manager (pointOfContact), Institute for Marine and Antarctic Studies (IMAS), University of Tasmania (UTAS) (hasAssociationWith), Kawaguchi, So (owner), Nichols, Peter (owner), Nicol, Stephen (owner), Virtue, Patti (hasPrincipalInvestigator)
• Format: Dataset
• Language: unknown
• Published: University of Tasmania, Australia
• Subjects: biota; Ocean acidification; Euphausia superba; EARTH SCIENCE | BIOLOGICAL CLASSIFICATION | ANIMALS/INVERTEBRATES | ARTHROPODS | CRUSTACEANS | EUPHAUSIIDS (KRILL); EARTH SCIENCE | AGRICULTURE | ANIMAL SCIENCE | ANIMAL PHYSIOLOGY AND BIOCHEMISTRY; Biological Oceanography; EARTH SCIENCES; OCEANOGRAPHY; Marine and Estuarine Ecology (incl. Marine Ichthyology); BIOLOGICAL SCIENCES; ECOLOGY; Ecosystem Function; ENVIRONMENTAL SCIENCES; ECOLOGICAL APPLICATIONS; Antarctic; Antarc*; aurora australis
• Online Access: https://researchdata.edu.au/krill-ocean-acidification-physiology-data/1728648

Maintenance and Update Frequency: notPlanned Statement: 4.3. Materials and Methods 4.3.1. Experimental conditions Live krill were collected on the RSV Aurora Australis via rectangular mid-water trawl on 22nd – 23rd February 2015 (66–03°S, 59–25°E and 66–33°S, 59–35°E). Krill were held in shipboard aquaria using standard maintenance methods (King et al. 2003) before being transferred to the Australian Antarctic Division’s (AAD) Krill Aquarium in Tasmania (seawater temperature 0.5°C and pH 8.1). Seawater was supplied to aquarium tanks via a seawater recirculating system (Kawaguchi et al. 2010). For ocean acidification experiments, 0.5°C seawater was supplied from a 70 L header tank and equilibrated with air (control) or CO2-enriched air (elevated pCO2 treatments) before delivery to experimental tanks. The CO2-enriched air was monitored using mass flow controllers (Horiba STEC SEC-E-40) and air valves, to regulate flow rates of atmospheric air and pure CO2. Five experimental 300 L tanks were maintained at five pCO2 levels; control 400 μatm pCO2 (pH 8.1), 1000 μatm pCO2 (pH 7.8), 1500 μatm pCO2 (pH 7.6), 2000 μatm pCO2 (pH 7.4) and 4000 μatm pCO2 (pH 7.1). Appropriate tank size and the best possible animal husbandry were high priorities in such a long-term study. As krill are a pelagic species, large sized (300 L) experimental tanks were needed to emulate wild conditions as closely as possible in a laboratory. Our experimental design was limited by the space and resources needed for these large tanks, and our observational units (CO2 treatment tanks) could not be replicated. We did not however, observe any visual evidence to suggest that ‘tank’ effects were confounding our results. Two hundred krill were randomly assigned to each experimental tank on 25th January 2016, corresponding to a density of 0.6 individuals L-1. This density is in the range of 0.5 – 2 individuals L-1 which has been successfully used in previous experiments at the AAD krill aquarium (Brown et al. 2013; Höring et al. 2018). The experiment ran ...