The role of ligand-gated chloride channels in behavioural alterations at elevated CO2 in a cephalopod

• Other Authors: Blake Spady (hasCollector), Jodi Thomas (hasCollector), Philip Laing Munday (hasCollector), Sue-Ann Watson (hasCollector)
• Format: Dataset
• Language: unknown
• Published: James Cook University
• Subjects: ocean acidification; squid; behaviour; GABA; ligand-gated ion channels; ligand-gated chloride channels; ionotropic receptors; gabazine; picrotoxin; carbon dioxide; cephalopod; ARC Centre of Excellence for Coral Reef Studies; Ocean acidification
• Online Access: https://researchdata.edu.au/the-role-ligand-co2-cephalopod/1711446; https://research.jcu.edu.au/data/published/3a248d4048d817dcf358b9d61b318a80; https://doi.org/10.25903/y6kz-hm11

Data from an experiment in which squid were exposed to ambient or elevated CO 2 levels, followed by treatment with sham, gabazine (GABA-A R antagonist) or picrotoxin (non-specific GABA-A R antagonist) and behavioural testing (mirror exposure). Two-toned pygmy squid (Idiosepius pygmaues) were collected from the wild and after 1-6 days of acclimation at ambient CO 2 levels were exposed to ambient (~450 µatm) or elevated (~1,000 µatm) CO 2 levels for 7 days. Squid were then treated with sham, 10.86 µM gabazine (GABA-A R antagonist) or 100 µM picrotoxin (non-specific GABA-A R antagonist) by bath application for 30 minutes, followed by a behavioural test; squid were placed in a square tank with a mirror along the length of one wall. Video recordings of each behavioural test were taken for approx. 16 minutes (introduction to the tank + 15 minute behavioural trial + tank approach). The behavioural videos were manually analysed for: • Proportion of squid that touched the mirror softly/aggressively • Latency to the first soft/aggressive mirror touch (only for squid that touched the mirror) • Number of soft/aggressive mirror touches – squid’s arms had to detach from the mirror completely between mirror touches before a successive touch was counted (only for squid that touched the mirror) Soft mirror touches were exploratory and defined as only the arm tips touching the mirror. Aggressive mirror touches occurred when the arms splayed upon coming in contact with the mirror, usually at high speed and accompanied by flashing body colour. Other parts of the squid’s body coming in contact with the mirror (e.g. mantle) were not counted as mirror touches. Tracking software was used to determine: • Time spent in Zone A (seconds) (only for squid that did enter Zone A) • Number of visits to Zone A • Time spent active (seconds) • Total distance moved (centimeters) • Average speed (cm/s) Zone A = a 3 cm wide zone along the full length of the mirror, created in LoliTrack. The effect of CO 2 and drug treatment on each of the measured ...