Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005)

• Other Authors: FERRARI, BELINDA C (hasPrincipalInvestigator), FERRARI, BELINDA C (processor), RAY, ANGELIQUE (processor), Australian Antarctic Data Centre (publisher)
• Format: Dataset
• Language: unknown
• Published: Australian Antarctic Data Centre
• Subjects: biota; MICROFAUNA; EARTH SCIENCE; LAND SURFACE; SOILS; MICROFLORA; METAGENOMIC; SOIL; MICROBIAL COMMUNITY ANALYSIS; SOIL MICROBIOME; ADS > Automated DNA Sequencer; FIELD SURVEYS; LABORATORY; GEOGRAPHIC REGION > POLAR; CONTINENT > ANTARCTICA > MITCHELL PENINSULA; BROWNING PENINSULA; WINDMILL ISLANDS; EAST ANTARCTICA; East Antarctica; Windmill Islands; Browning; Browning Peninsula; Mitchell Peninsula; Antarc*; Antarctica
• Online Access: https://researchdata.ands.org.au/metagenomic-browning-peninsula-sampled-2005/1442021; https://doi.org/10.26179/5e33a1e1e8c87; https://data.aad.gov.au/metadata/records/AAS_4406_BP_MP_2005; http://nla.gov.au/nla.party-617536

Sample collection: All soils were collected in 2005 and stored at -80˚C prior to gDNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, NSW, Australia). Three soil samples were obtained from Mitchell Peninsula (66°31'S, 110°59'E) and three were obtained from Browning Peninsula (66°27'S, 110°32'E). Samples from both sites were selected along a transect, with specific information provided below. Metagenome sequencing and assembly: Metagenomic shotgun libraries were prepared from community gDNA extractions using the Nextera DNA Flex Library Prep Kit (Illumina Inc.). Sequencing was performed on an Illumina NextSeq500 platform with 2×150bp base pair high output run chemistry and 5GB coverage per sample. Description of ACE Sample IDs (including in filenames) and the relevant soil samples: * SB9748 - Soil barcode= 91062 - Original barcode= 91604 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 2m - Concentration of DNA sent= 1.2ng/µL (required concentration) - Volume DNA sent= 80µL * SB9749 - Soil barcode= 91604 - Original barcode= 26587 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 102m - Concentration of DNA sent= 1ng/µL (required concentration) - Volume DNA sent= 80µL * SB9750 - Soil barcode= 91606 - Original barcode= 26617 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 202m - Concentration of DNA sent= 0.3ng/µL (required concentration) - Volume DNA sent= 180µL * SB9751 - Soil barcode= 91447 - Original barcode= 36260 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 2m - Concentration of DNA sent= 0.86ng/µL (required concentration) - Volume DNA sent= 80µL * SB9752 - Soil barcode= 91477 - Original barcode= 36290 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 102m - Concentration of DNA sent= 0.9ng/µL (required concentration) - Volume DNA sent= 80µL * SB9753 - Soil barcode= 91507 - Original barcode= 36320 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 202m - Concentration of DNA sent= 1.9ng/µL (required concentration) - Volume DNA sent= 80µL