Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005)
Sample collection: All soils were collected in 2005 and stored at -80˚C prior to gDNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, NSW, Australia). Three soil samples were obtained from Mitchell Peninsula (66°31'S, 110°59'E) and three were obtained from Browning Peninsul...
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Australian Antarctic Data Centre
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Online Access: | https://researchdata.ands.org.au/metagenomic-browning-peninsula-sampled-2005/1442021 https://doi.org/10.26179/5e33a1e1e8c87 https://data.aad.gov.au/metadata/records/AAS_4406_BP_MP_2005 http://nla.gov.au/nla.party-617536 |
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ftands:oai:ands.org.au::1442021 2023-05-15T13:52:00+02:00 Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005) FERRARI, BELINDA C (hasPrincipalInvestigator) FERRARI, BELINDA C (processor) RAY, ANGELIQUE (processor) Australian Antarctic Data Centre (publisher) Spatial: northlimit=-66.20709; southlimit=-66.38156; westlimit=110.42908; eastLimit=110.68176; projection=WGS84 Temporal: From 2005-01-01 to 2005-12-31 https://researchdata.ands.org.au/metagenomic-browning-peninsula-sampled-2005/1442021 https://doi.org/10.26179/5e33a1e1e8c87 https://data.aad.gov.au/metadata/records/AAS_4406_BP_MP_2005 http://nla.gov.au/nla.party-617536 unknown Australian Antarctic Data Centre https://researchdata.ands.org.au/metagenomic-browning-peninsula-sampled-2005/1442021 6b9bf7da-74bc-45a7-b175-6ebe283e5a94 doi:10.26179/5e33a1e1e8c87 AAS_4406_BP_MP_2005 https://data.aad.gov.au/metadata/records/AAS_4406_BP_MP_2005 http://nla.gov.au/nla.party-617536 Australian Antarctic Data Centre biota MICROFAUNA EARTH SCIENCE LAND SURFACE SOILS MICROFLORA METAGENOMIC SOIL MICROBIAL COMMUNITY ANALYSIS SOIL MICROBIOME ADS > Automated DNA Sequencer FIELD SURVEYS LABORATORY GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > MITCHELL PENINSULA BROWNING PENINSULA WINDMILL ISLANDS EAST ANTARCTICA dataset ftands https://doi.org/10.26179/5e33a1e1e8c87 2020-02-03T23:24:56Z Sample collection: All soils were collected in 2005 and stored at -80˚C prior to gDNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, NSW, Australia). Three soil samples were obtained from Mitchell Peninsula (66°31'S, 110°59'E) and three were obtained from Browning Peninsula (66°27'S, 110°32'E). Samples from both sites were selected along a transect, with specific information provided below. Metagenome sequencing and assembly: Metagenomic shotgun libraries were prepared from community gDNA extractions using the Nextera DNA Flex Library Prep Kit (Illumina Inc.). Sequencing was performed on an Illumina NextSeq500 platform with 2×150bp base pair high output run chemistry and 5GB coverage per sample. Description of ACE Sample IDs (including in filenames) and the relevant soil samples: * SB9748 - Soil barcode= 91062 - Original barcode= 91604 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 2m - Concentration of DNA sent= 1.2ng/µL (required concentration) - Volume DNA sent= 80µL * SB9749 - Soil barcode= 91604 - Original barcode= 26587 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 102m - Concentration of DNA sent= 1ng/µL (required concentration) - Volume DNA sent= 80µL * SB9750 - Soil barcode= 91606 - Original barcode= 26617 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 202m - Concentration of DNA sent= 0.3ng/µL (required concentration) - Volume DNA sent= 180µL * SB9751 - Soil barcode= 91447 - Original barcode= 36260 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 2m - Concentration of DNA sent= 0.86ng/µL (required concentration) - Volume DNA sent= 80µL * SB9752 - Soil barcode= 91477 - Original barcode= 36290 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 102m - Concentration of DNA sent= 0.9ng/µL (required concentration) - Volume DNA sent= 80µL * SB9753 - Soil barcode= 91507 - Original barcode= 36320 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 202m - Concentration of DNA sent= 1.9ng/µL (required concentration) - Volume DNA sent= 80µL Dataset Antarc* Antarctica East Antarctica Windmill Islands Research Data Australia (Australian National Data Service - ANDS) East Antarctica Windmill Islands ENVELOPE(110.417,110.417,-66.350,-66.350) Browning ENVELOPE(164.050,164.050,-74.617,-74.617) Browning Peninsula ENVELOPE(110.550,110.550,-66.469,-66.469) Mitchell Peninsula ENVELOPE(110.548,110.548,-66.328,-66.328) ENVELOPE(110.42908,110.68176,-66.20709,-66.38156) |
institution |
Open Polar |
collection |
Research Data Australia (Australian National Data Service - ANDS) |
op_collection_id |
ftands |
language |
unknown |
topic |
biota MICROFAUNA EARTH SCIENCE LAND SURFACE SOILS MICROFLORA METAGENOMIC SOIL MICROBIAL COMMUNITY ANALYSIS SOIL MICROBIOME ADS > Automated DNA Sequencer FIELD SURVEYS LABORATORY GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > MITCHELL PENINSULA BROWNING PENINSULA WINDMILL ISLANDS EAST ANTARCTICA |
spellingShingle |
biota MICROFAUNA EARTH SCIENCE LAND SURFACE SOILS MICROFLORA METAGENOMIC SOIL MICROBIAL COMMUNITY ANALYSIS SOIL MICROBIOME ADS > Automated DNA Sequencer FIELD SURVEYS LABORATORY GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > MITCHELL PENINSULA BROWNING PENINSULA WINDMILL ISLANDS EAST ANTARCTICA Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005) |
topic_facet |
biota MICROFAUNA EARTH SCIENCE LAND SURFACE SOILS MICROFLORA METAGENOMIC SOIL MICROBIAL COMMUNITY ANALYSIS SOIL MICROBIOME ADS > Automated DNA Sequencer FIELD SURVEYS LABORATORY GEOGRAPHIC REGION > POLAR CONTINENT > ANTARCTICA > MITCHELL PENINSULA BROWNING PENINSULA WINDMILL ISLANDS EAST ANTARCTICA |
description |
Sample collection: All soils were collected in 2005 and stored at -80˚C prior to gDNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, NSW, Australia). Three soil samples were obtained from Mitchell Peninsula (66°31'S, 110°59'E) and three were obtained from Browning Peninsula (66°27'S, 110°32'E). Samples from both sites were selected along a transect, with specific information provided below. Metagenome sequencing and assembly: Metagenomic shotgun libraries were prepared from community gDNA extractions using the Nextera DNA Flex Library Prep Kit (Illumina Inc.). Sequencing was performed on an Illumina NextSeq500 platform with 2×150bp base pair high output run chemistry and 5GB coverage per sample. Description of ACE Sample IDs (including in filenames) and the relevant soil samples: * SB9748 - Soil barcode= 91062 - Original barcode= 91604 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 2m - Concentration of DNA sent= 1.2ng/µL (required concentration) - Volume DNA sent= 80µL * SB9749 - Soil barcode= 91604 - Original barcode= 26587 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 102m - Concentration of DNA sent= 1ng/µL (required concentration) - Volume DNA sent= 80µL * SB9750 - Soil barcode= 91606 - Original barcode= 26617 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 202m - Concentration of DNA sent= 0.3ng/µL (required concentration) - Volume DNA sent= 180µL * SB9751 - Soil barcode= 91447 - Original barcode= 36260 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 2m - Concentration of DNA sent= 0.86ng/µL (required concentration) - Volume DNA sent= 80µL * SB9752 - Soil barcode= 91477 - Original barcode= 36290 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 102m - Concentration of DNA sent= 0.9ng/µL (required concentration) - Volume DNA sent= 80µL * SB9753 - Soil barcode= 91507 - Original barcode= 36320 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 202m - Concentration of DNA sent= 1.9ng/µL (required concentration) - Volume DNA sent= 80µL |
author2 |
FERRARI, BELINDA C (hasPrincipalInvestigator) FERRARI, BELINDA C (processor) RAY, ANGELIQUE (processor) Australian Antarctic Data Centre (publisher) |
format |
Dataset |
title |
Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005) |
title_short |
Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005) |
title_full |
Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005) |
title_fullStr |
Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005) |
title_full_unstemmed |
Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005) |
title_sort |
metagenomic data for browning peninsula and mitchell peninsula (sampled 2005) |
publisher |
Australian Antarctic Data Centre |
url |
https://researchdata.ands.org.au/metagenomic-browning-peninsula-sampled-2005/1442021 https://doi.org/10.26179/5e33a1e1e8c87 https://data.aad.gov.au/metadata/records/AAS_4406_BP_MP_2005 http://nla.gov.au/nla.party-617536 |
op_coverage |
Spatial: northlimit=-66.20709; southlimit=-66.38156; westlimit=110.42908; eastLimit=110.68176; projection=WGS84 Temporal: From 2005-01-01 to 2005-12-31 |
long_lat |
ENVELOPE(110.417,110.417,-66.350,-66.350) ENVELOPE(164.050,164.050,-74.617,-74.617) ENVELOPE(110.550,110.550,-66.469,-66.469) ENVELOPE(110.548,110.548,-66.328,-66.328) ENVELOPE(110.42908,110.68176,-66.20709,-66.38156) |
geographic |
East Antarctica Windmill Islands Browning Browning Peninsula Mitchell Peninsula |
geographic_facet |
East Antarctica Windmill Islands Browning Browning Peninsula Mitchell Peninsula |
genre |
Antarc* Antarctica East Antarctica Windmill Islands |
genre_facet |
Antarc* Antarctica East Antarctica Windmill Islands |
op_source |
Australian Antarctic Data Centre |
op_relation |
https://researchdata.ands.org.au/metagenomic-browning-peninsula-sampled-2005/1442021 6b9bf7da-74bc-45a7-b175-6ebe283e5a94 doi:10.26179/5e33a1e1e8c87 AAS_4406_BP_MP_2005 https://data.aad.gov.au/metadata/records/AAS_4406_BP_MP_2005 http://nla.gov.au/nla.party-617536 |
op_doi |
https://doi.org/10.26179/5e33a1e1e8c87 |
_version_ |
1766256130852388864 |