Metagenomic Data for Browning Peninsula and Mitchell Peninsula (sampled 2005)

Sample collection: All soils were collected in 2005 and stored at -80˚C prior to gDNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, NSW, Australia). Three soil samples were obtained from Mitchell Peninsula (66°31'S, 110°59'E) and three were obtained from Browning Peninsul...

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Bibliographic Details
Other Authors: FERRARI, BELINDA C (hasPrincipalInvestigator), FERRARI, BELINDA C (processor), RAY, ANGELIQUE (processor), Australian Antarctic Data Centre (publisher)
Format: Dataset
Language:unknown
Published: Australian Antarctic Data Centre
Subjects:
biota
MICROFAUNA
EARTH SCIENCE
LAND SURFACE
SOILS
MICROFLORA
METAGENOMIC
SOIL
MICROBIAL COMMUNITY ANALYSIS
SOIL MICROBIOME
ADS &gt
Automated DNA Sequencer
FIELD SURVEYS
LABORATORY
GEOGRAPHIC REGION &gt
POLAR
CONTINENT &gt
ANTARCTICA &gt
MITCHELL PENINSULA
BROWNING PENINSULA
WINDMILL ISLANDS
EAST ANTARCTICA
East Antarctica
Windmill Islands
Browning
Browning Peninsula
Mitchell Peninsula
Antarc*
Antarctica
Online Access:https://researchdata.ands.org.au/metagenomic-browning-peninsula-sampled-2005/1442021
https://doi.org/10.26179/5e33a1e1e8c87
https://data.aad.gov.au/metadata/records/AAS_4406_BP_MP_2005
http://nla.gov.au/nla.party-617536
Description
Summary:Sample collection: All soils were collected in 2005 and stored at -80˚C prior to gDNA extraction using the FastDNA SPIN kit for soil (MP Biomedicals, NSW, Australia). Three soil samples were obtained from Mitchell Peninsula (66°31'S, 110°59'E) and three were obtained from Browning Peninsula (66°27'S, 110°32'E). Samples from both sites were selected along a transect, with specific information provided below. Metagenome sequencing and assembly: Metagenomic shotgun libraries were prepared from community gDNA extractions using the Nextera DNA Flex Library Prep Kit (Illumina Inc.). Sequencing was performed on an Illumina NextSeq500 platform with 2×150bp base pair high output run chemistry and 5GB coverage per sample. Description of ACE Sample IDs (including in filenames) and the relevant soil samples: * SB9748 - Soil barcode= 91062 - Original barcode= 91604 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 2m - Concentration of DNA sent= 1.2ng/µL (required concentration) - Volume DNA sent= 80µL * SB9749 - Soil barcode= 91604 - Original barcode= 26587 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 102m - Concentration of DNA sent= 1ng/µL (required concentration) - Volume DNA sent= 80µL * SB9750 - Soil barcode= 91606 - Original barcode= 26617 - Sample Site= Browning Peninsula - Transect= 3 - Distance samples= 202m - Concentration of DNA sent= 0.3ng/µL (required concentration) - Volume DNA sent= 180µL * SB9751 - Soil barcode= 91447 - Original barcode= 36260 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 2m - Concentration of DNA sent= 0.86ng/µL (required concentration) - Volume DNA sent= 80µL * SB9752 - Soil barcode= 91477 - Original barcode= 36290 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 102m - Concentration of DNA sent= 0.9ng/µL (required concentration) - Volume DNA sent= 80µL * SB9753 - Soil barcode= 91507 - Original barcode= 36320 - Sample Site= Mitchell Peninsula - Transect= 2 - Distance samples= 202m - Concentration of DNA sent= 1.9ng/µL (required concentration) - Volume DNA sent= 80µL

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