Invasion and replication of Yersinia ruckeri in fish cell cultures
Background: Like many members of the Enterobacteriaceae family, Yersinia ruckeri has the ability to invade non professional phagocytic cells. Intracellular location is advantageous for the bacterium because it shields it from the immune system and can help it cross epithelial membranes and gain entr...
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Online Access: | https://vbn.aau.dk/da/publications/4823a841-c8cb-4abf-906c-161e347691cd https://doi.org/10.1186/s12917-018-1408-1 http://www.scopus.com/inward/record.url?scp=85043470226&partnerID=8YFLogxK |
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ftalborgunivpubl:oai:pure.atira.dk:publications/4823a841-c8cb-4abf-906c-161e347691cd 2024-06-23T07:51:20+00:00 Invasion and replication of Yersinia ruckeri in fish cell cultures Menanteau-Ledouble, Simon Lawrence, Mark L. El-Matbouli, Mansour 2018-03-09 https://vbn.aau.dk/da/publications/4823a841-c8cb-4abf-906c-161e347691cd https://doi.org/10.1186/s12917-018-1408-1 http://www.scopus.com/inward/record.url?scp=85043470226&partnerID=8YFLogxK eng eng https://vbn.aau.dk/da/publications/4823a841-c8cb-4abf-906c-161e347691cd info:eu-repo/semantics/openAccess Menanteau-Ledouble , S , Lawrence , M L & El-Matbouli , M 2018 , ' Invasion and replication of Yersinia ruckeri in fish cell cultures ' , BMC Veterinary Research , vol. 14 , no. 1 , 81 . https://doi.org/10.1186/s12917-018-1408-1 Atlantic Salmon kidney cells Chinook Salmon embryo cells Gentamycin assay Intracellular invasion Salmon head kidney cells article 2018 ftalborgunivpubl https://doi.org/10.1186/s12917-018-1408-1 2024-06-03T15:12:28Z Background: Like many members of the Enterobacteriaceae family, Yersinia ruckeri has the ability to invade non professional phagocytic cells. Intracellular location is advantageous for the bacterium because it shields it from the immune system and can help it cross epithelial membranes and gain entry into the host. In the present manuscript, we report on our investigation regarding the mechanisms of Y. ruckeri's invasion of host cells. Results: A gentamycin assay was applied to two isolates, belonging to both the biotype 1 (ATCC 29473) and biotype 2 (A7959-11) and using several cell culture types: Atlantic Salmon Kidney, Salmon Head Kidney and, Chinook salmon embryos cells at both low and high passage numbers. Varying degrees of sensitivity to Y. ruckeri infection were found between the cell types and the biotype 1 strain was found to be more invasive than the non-motile biotype 2 isolate. Furthermore, the effect of six chemical compounds (Cytochalasin D, TAE 226, vinblastine, genistein, colchicine and, N-acetylcysteine), known to interfere with bacterial invasion strategies, were investigated. All of these compounds had a significant impact on the ability of the bacterium to invade host cells. Changes in the concentration of bacterial cells over time were investigated and the results suggested that neither isolate could survive intracellularly for sustained periods. Conclusions: These results suggest that Y. ruckeri can gain entrance into host cells through several mechanisms, and might take advantage of both the actin and microtubule cytoskeletal systems. Article in Journal/Newspaper Atlantic salmon Aalborg University's Research Portal BMC Veterinary Research 14 1 |
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Open Polar |
collection |
Aalborg University's Research Portal |
op_collection_id |
ftalborgunivpubl |
language |
English |
topic |
Atlantic Salmon kidney cells Chinook Salmon embryo cells Gentamycin assay Intracellular invasion Salmon head kidney cells |
spellingShingle |
Atlantic Salmon kidney cells Chinook Salmon embryo cells Gentamycin assay Intracellular invasion Salmon head kidney cells Menanteau-Ledouble, Simon Lawrence, Mark L. El-Matbouli, Mansour Invasion and replication of Yersinia ruckeri in fish cell cultures |
topic_facet |
Atlantic Salmon kidney cells Chinook Salmon embryo cells Gentamycin assay Intracellular invasion Salmon head kidney cells |
description |
Background: Like many members of the Enterobacteriaceae family, Yersinia ruckeri has the ability to invade non professional phagocytic cells. Intracellular location is advantageous for the bacterium because it shields it from the immune system and can help it cross epithelial membranes and gain entry into the host. In the present manuscript, we report on our investigation regarding the mechanisms of Y. ruckeri's invasion of host cells. Results: A gentamycin assay was applied to two isolates, belonging to both the biotype 1 (ATCC 29473) and biotype 2 (A7959-11) and using several cell culture types: Atlantic Salmon Kidney, Salmon Head Kidney and, Chinook salmon embryos cells at both low and high passage numbers. Varying degrees of sensitivity to Y. ruckeri infection were found between the cell types and the biotype 1 strain was found to be more invasive than the non-motile biotype 2 isolate. Furthermore, the effect of six chemical compounds (Cytochalasin D, TAE 226, vinblastine, genistein, colchicine and, N-acetylcysteine), known to interfere with bacterial invasion strategies, were investigated. All of these compounds had a significant impact on the ability of the bacterium to invade host cells. Changes in the concentration of bacterial cells over time were investigated and the results suggested that neither isolate could survive intracellularly for sustained periods. Conclusions: These results suggest that Y. ruckeri can gain entrance into host cells through several mechanisms, and might take advantage of both the actin and microtubule cytoskeletal systems. |
format |
Article in Journal/Newspaper |
author |
Menanteau-Ledouble, Simon Lawrence, Mark L. El-Matbouli, Mansour |
author_facet |
Menanteau-Ledouble, Simon Lawrence, Mark L. El-Matbouli, Mansour |
author_sort |
Menanteau-Ledouble, Simon |
title |
Invasion and replication of Yersinia ruckeri in fish cell cultures |
title_short |
Invasion and replication of Yersinia ruckeri in fish cell cultures |
title_full |
Invasion and replication of Yersinia ruckeri in fish cell cultures |
title_fullStr |
Invasion and replication of Yersinia ruckeri in fish cell cultures |
title_full_unstemmed |
Invasion and replication of Yersinia ruckeri in fish cell cultures |
title_sort |
invasion and replication of yersinia ruckeri in fish cell cultures |
publishDate |
2018 |
url |
https://vbn.aau.dk/da/publications/4823a841-c8cb-4abf-906c-161e347691cd https://doi.org/10.1186/s12917-018-1408-1 http://www.scopus.com/inward/record.url?scp=85043470226&partnerID=8YFLogxK |
genre |
Atlantic salmon |
genre_facet |
Atlantic salmon |
op_source |
Menanteau-Ledouble , S , Lawrence , M L & El-Matbouli , M 2018 , ' Invasion and replication of Yersinia ruckeri in fish cell cultures ' , BMC Veterinary Research , vol. 14 , no. 1 , 81 . https://doi.org/10.1186/s12917-018-1408-1 |
op_relation |
https://vbn.aau.dk/da/publications/4823a841-c8cb-4abf-906c-161e347691cd |
op_rights |
info:eu-repo/semantics/openAccess |
op_doi |
https://doi.org/10.1186/s12917-018-1408-1 |
container_title |
BMC Veterinary Research |
container_volume |
14 |
container_issue |
1 |
_version_ |
1802642399927730176 |