New efficient recombinant expression system to engineer Candida antarctica lipase B

• Published in: Applied and Environmental Microbiology
• Main Authors: Emond, Stéphane, Montanier, Cédric, Nicaud, Jean-Marc, J.-M., Marty, Alain, A., Monsan, Pierre, André, Isabelle, Remaud Simeon, Magali
• Other Authors: Laboratoire d'Ingénierie des Systèmes Biologiques et des Procédés (LISBP), Institut National de la Recherche Agronomique (INRA)-Institut National des Sciences Appliquées - Toulouse (INSA Toulouse), Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Institut National des Sciences Appliquées (INSA)-Université de Toulouse (UT)-Centre National de la Recherche Scientifique (CNRS), Microbiologie et Génétique Moléculaire (MGM), Institut National de la Recherche Agronomique (INRA)-Institut National Agronomique Paris-Grignon (INA P-G)-Centre National de la Recherche Scientifique (CNRS), Institut universitaire de France (IUF), Ministère de l'Education nationale, de l’Enseignement supérieur et de la Recherche (M.E.N.E.S.R.), French National Research Agency ANR-07CP2D-15
• Format: Article in Journal/Newspaper
• Language: English
• Published: HAL CCSD 2010
• Subjects: [SDV.BIO]Life Sciences [q-bio]/Biotechnology; Antarc*; Antarctica
• Online Access: https://hal.inrae.fr/hal-02662242; https://doi.org/10.1128/AEM.03057-09

International audience Here, we report the use of Yarrowia lipolytica as a versatile expression host for developing protein engineering approaches to modify the properties of Candida antarctica lipase B. A reliable screening protocol was defined and validated using a saturation mutagenesis library, yielding mutants displaying higher catalytic efficiencies than the wild-type enzyme.