| Summary: | The nutrients and carbon sources used in cellular agriculture result in high continuous expenses which in turn slow down the interest of utilizing in plant cell culture -based products in food industry. Sucrose is the most used carbon source for plant cell cultures. It is also the most expensive single ingredient in plant cell culture medium. In industries, and especially in food production, large amounts of nutrient and sugar-rich side streams are generated, which the producers would like to valorize more effectively. The aim for this study was to evaluate the usability of industrial side streams as carbon and nutrient source for plant cell cultures. In the experiments the composition of nine different side stream samples S1 – S9 were analyzed and their usability to replace sucrose and nutrients in the traditional plant cell growth medium was evaluated. Based on the anal-yses, side streams S4 – S7 were determined the most suitable supplements for plant cell culture medium, of which S5 – S7 were used as a source of car-bon and side stream S4 as a nutrient source due to its high salt and nutrient content. Side stream supplementation was examined in suspension cultures of arctic bramble, lingonberry, silver birch, and tobacco. Based on previous studies, plant cells can utilize various mono- and disaccharides as a carbon source, but there are species differences in the utilization abilities. The effect of side stream supplementation on plant cell culture growth was assessed by comparing biomass, appearance, and viability of the cultures. The experiments confirmed that there are species-specific differences in the utilization ability of different carbohydrates. Arctic bramble cells were able to use side stream S6 as a carbon source and possibly adapt to utilize S7 after two cultivation cycles. Side streams S6 and S7 were found to increase lingonberry biomass production compared to the corresponding control, but the cells did not utilize the sugars contained in S7. In addition, the side stream S4 was successfully ...
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