TN249-10 - Lipofuscin and Euphausiid Aging

Age determination in crustaceans relies on quantification of a fluorescent age pigment, lipofuscin, that accumulates in eye stalks of krill over time. Lipofuscin includes a mixture of protein oxidation products formed in neural tissues. The extracted products are quantified using a fluorescence dete...

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Bibliographic Details
Main Authors: H. Rodger Harvey, Rachel Pleuthner
Format: Dataset
Language:unknown
Published: Arctic Data Center
Subjects:
Online Access:https://doi.org/10.5065/D69P2ZPX
Description
Summary:Age determination in crustaceans relies on quantification of a fluorescent age pigment, lipofuscin, that accumulates in eye stalks of krill over time. Lipofuscin includes a mixture of protein oxidation products formed in neural tissues. The extracted products are quantified using a fluorescence detector. The concentration of lipofuscin is normalized to the protein content from the same tissues. The ratio of lipofuscin to protein concentration is the Lipofuscin Index and is calculated for each individual organism. In order for the index to reflect chronological age, a calibration is required using euphausiids of known age. A comparison of the calculated ages to the specific lengths for each krill provides the ages for those krill analyzed during each cruise. These data were collected aboard the UNOLS R/V Thompson ship.