Genetic discrimination of PWS Herring populations: 1994-1998

We examined spatial and temporal patternsof genetic variation at five microsatellite loci and one mtDNA gene in seven spawning aggregations of Pacific herri(nCglupeaharengus) sampled in 1995 and 1996. Sample locations included Prince William Sound (4 sites) and Kodiak Island(1site)intheGulfof Alaska...

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Bibliographic Details
Main Authors: Jeffrey Olsen, Paul Bentzen, Jonathan Wright, Jim Seeb, Lisa Seeb, Susan Merkouris
Format: Dataset
Language:unknown
Published: Gulf of Alaska Data Portal
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Online Access:https://search.dataone.org/view/df35a.38.3
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Summary:We examined spatial and temporal patternsof genetic variation at five microsatellite loci and one mtDNA gene in seven spawning aggregations of Pacific herri(nCglupeaharengus) sampled in 1995 and 1996. Sample locations included Prince William Sound (4 sites) and Kodiak Island(1site)intheGulfof Alaska, and Togiak Bay (1 site) and Norton Sound (1 site) in the Bering Sea. An analysis of molecular variance revealed a marked genetic discontinuity between herring in the Bering Sea and Gulfof Alaska. The estimatesof genetic differentiation between populations from the two sea basins (F_BT) were significant (P<0.001) and ranged from 0.169 for mtDNA to 0.209 for microsatellites. The estimates of genetic differentiation within sea basins (F_SB) were 0.013 for mtDNA and 0.030f or microsatellites and were significant (P<0.001). The microsatellites also showed significant (P<0.001) temporal variation within the Gulf of Alaska. The estimates of genetic variation between years at each sample location(F_SL) were 0.011 formicrosatellites and 0.012 for mtDNA. The temporal shifts in genetic structure may reflect large inter-annual variation in reproductive success and hamper the use of genetic markers for discrete stock management of herring in Prince William Sound. This multi-year study was initiated in FY94 as Restoration Project 94165. However, a return failure of herring in Prince William Sound in 1994 delayed implementation until FY95. During the first two years (FY95, FY96) new molecular markers were developed and tested for population genetic analyses. In addition, herring samples were collected from spawning aggregations within Prince William Sound (4 sites), Kodiak Island(1 site), TogiakBay (1 site), and Norton Sound(1 site). During the final two years (FY97, FY98) samples were genotyped and genetic data were analyzed. Preliminary results were reported to the Exxon Valdez Oil Spill (EVOS)Trustee Council and EVOS project reviewers in a poster session at the January1996 EVOS workshop in Anchorage. A synthesis of the herring genetic data was reported to the EVOS peer reviewers in February 1998. Four reports were submitted consulting scientists detailing the development and application of mtDNA and microsatellite markers. One progress report was submitted by the principal investigators in 1996 under the title Genetic Discrimination of Prince William Sound Herring Populations. In total, 2,117,781 raw sequencing reads were generated with an average length of 254 bp. All data was submitted to NBCI’s Short Read Archive under accession number SRX022719. This can be found at http://www.ncbi.nlm.nih.gov/sra/?term=SRX022719 .