| Summary: | Microbial communities colonizing Pandalus borealis (the northern shrimp) plays an important role in their ecosystem. Although Pandalus borealis attracts extensive attention worldwide for a long time, the microbial community and composition remain unclear. Therefore, the microbial diversity and community composition were studied by high-throughput sequencing technology and traditional cultured method. Whole shrimp were dissected into components including eyes, shrimp eggs, viscera, muscles, and shrimp tails to evaluate the diversity of culture-independent microorganisms. The results showed that the microbial composition of different body parts were similar, although fungal and bacterial community diversity of the viscera were highest. The most predominant bacterial phylum was the Proteobacteria that accounted for 13.47–89.82% of all reads from different body parts, except for shrimp eggs. Only the CXQ1 whole-shrimp and viscera of CXQ25 yielded fungal community results, of which the predominant phylum and genus were Acomycota (48.02% and 71.21% in CXQ1 and CXQ25, respectively) and Aspergillus (4.52% and 4.18%), respectively. Moreover, bacterial functional predictions and fungal guild were also investigated on the basis of OTUs results. Meanwhile, the traditional cultivable method showed that total 22 Acomycota phylum fungal colonies were identified by 18S rRNA sequencing technology. These findings provide new insights for culturable and inculturable microbial community composition of Pandalus borealis for the first time.
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