Shared and nonshared genomic divergence in parallel ecotypes of Littorina saxatilis at a local scale

• Published in: Molecular Ecology
• Main Authors: Ravinet, Mark, Westram, Anja, Johannesson, Kerstin, Butlin, Roger, André, Carl, Panova, Marina
• Other Authors: Vetenskapsrådet, Natural Environment Research Council, Linnaeus Centre for Marine Evolutionary Biology
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2015
• Subjects: Close Islands
• Online Access: http://dx.doi.org/10.1111/mec.13332; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fmec.13332; https://onlinelibrary.wiley.com/doi/pdf/10.1111/mec.13332

Abstract Parallel speciation occurs when selection drives repeated, independent adaptive divergence that reduces gene flow between ecotypes. Classical examples show parallel speciation originating from shared genomic variation, but this does not seem to be the case in the rough periwinkle ( L ittorina saxatilis ) that has evolved considerable phenotypic diversity across Europe, including several distinct ecotypes. Small ‘wave’ ecotype snails inhabit exposed rocks and experience strong wave action, while thick‐shelled, ‘crab’ ecotype snails are larger and experience crab predation on less exposed shores. Crab and wave ecotypes appear to have arisen in parallel, and recent evidence suggests only marginal sharing of molecular variation linked to evolution of similar ecotypes in different parts of Europe. However, the extent of genomic sharing is expected to increase with gene flow and more recent common ancestry. To test this, we used de novo RAD ‐sequencing to quantify the extent of shared genomic divergence associated with phenotypic similarities amongst ecotype pairs on three close islands (<10 km distance) connected by weak gene flow ( Nm ~ 0.03) and with recent common ancestry (<10 000 years). After accounting for technical issues, including a large proportion of null alleles due to a large effective population size, we found ~8–28% of positive outliers were shared between two islands and ~2–9% were shared amongst all three islands. This low level of sharing suggests that parallel phenotypic divergence in this system is not matched by shared genomic divergence despite a high probability of gene flow and standing genetic variation.