Laser‐capture dissection and immunohistochemistry reveals chloride and mucous‐cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar

• Published in: Journal of Fish Biology
• Main Authors: Nowak, B., Cadoret, K., Feist, S.W., Bean, T.P.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2013
• Subjects: Aquatic Science; Ecology, Evolution, Behavior and Systematics; Atlantic salmon; Salmo salar
• Online Access: http://dx.doi.org/10.1111/jfb.12235; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfb.12235; https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.12235

Laser‐capture microdissection and immunohistochemistry were used to show that gene and protein expression varied in different cell types in the gills of Atlantic salmon Salmo salar , with chloride cells found to express high levels of sodium potassium ATPase and mucous cells expressing elevated levels of anterior gradient protein. It is therefore important that studies of gene expression in gill tissue take account of the proportion of the various cell types present.