Laser‐capture dissection and immunohistochemistry reveals chloride and mucous‐cell specific gene expression in gills of seawater acclimated Atlantic salmon Salmo salar

Laser‐capture microdissection and immunohistochemistry were used to show that gene and protein expression varied in different cell types in the gills of Atlantic salmon Salmo salar , with chloride cells found to express high levels of sodium potassium ATPase and mucous cells expressing elevated leve...

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Bibliographic Details
Published in:Journal of Fish Biology
Main Authors: Nowak, B., Cadoret, K., Feist, S.W., Bean, T.P.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2013
Subjects:
Aquatic Science
Ecology, Evolution, Behavior and Systematics
Atlantic salmon
Salmo salar
Online Access:http://dx.doi.org/10.1111/jfb.12235
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fjfb.12235
https://onlinelibrary.wiley.com/doi/pdf/10.1111/jfb.12235
Description
Summary:Laser‐capture microdissection and immunohistochemistry were used to show that gene and protein expression varied in different cell types in the gills of Atlantic salmon Salmo salar , with chloride cells found to express high levels of sodium potassium ATPase and mucous cells expressing elevated levels of anterior gradient protein. It is therefore important that studies of gene expression in gill tissue take account of the proportion of the various cell types present.

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