Novel set of multiplex assays (SalPrint15) for efficient analysis of 15 microsatellite loci of contemporary samples of the Atlantic salmon ( Salmo salar )

Abstract We report the development of new PCR assays and loading panels for the Atlantic salmon using 15 microsatellite loci. A total of 8, 3 and 4 loci were coamplified in three separate PCRs using labelled primers and loaded on the ABI DNA analyzer in two separate panels. Amplified alleles were cl...

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Bibliographic Details
Published in:Molecular Ecology Resources
Main Authors: OLAFSSON, KRISTINN, HJORLEIFSDOTTIR, SIGRIDUR, PAMPOULIE, CHRISTOPHE, HREGGVIDSSON, GUDMUNDUR OLI, GUDJONSSON, SIGURDUR
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2010
Subjects:
Genetics
Ecology, Evolution, Behavior and Systematics
Biotechnology
Atlantic salmon
Salmo salar
Online Access:http://dx.doi.org/10.1111/j.1755-0998.2009.02781.x
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https://onlinelibrary.wiley.com/doi/full-xml/10.1111/j.1755-0998.2009.02781.x
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Summary:Abstract We report the development of new PCR assays and loading panels for the Atlantic salmon using 15 microsatellite loci. A total of 8, 3 and 4 loci were coamplified in three separate PCRs using labelled primers and loaded on the ABI DNA analyzer in two separate panels. Amplified alleles were clearly typed, and easily interpretable results were obtained. The method was successfully applied in different laboratories, even when different types of DNA polymerase were employed. The method is useful for analysing paternity, population genetics and conservation as well as for selective breeding programmes.

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