Characterization of 155 EST‐derived microsatellites from Atlantic cod ( Gadus morhua ) and validation for linkage mapping
Abstract Microsatellite markers for Atlantic cod ( Gadus morhua ) were identified from a collection of 30 630 expressed sequence tags. Primers were designed for 395 microsatellites and 155 were successfully amplified. Allele number varied from 1 to 26 (average 6.34). Average observed and expected he...
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crwiley:10.1111/j.1755-0998.2008.02475.x 2023-12-03T10:18:54+01:00 Characterization of 155 EST‐derived microsatellites from Atlantic cod ( Gadus morhua ) and validation for linkage mapping HIGGINS, B. HUBERT, S. SIMPSON, G. STONE, C. BOWMAN, S. 2009 http://dx.doi.org/10.1111/j.1755-0998.2008.02475.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2008.02475.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2008.02475.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor Molecular Ecology Resources volume 9, issue 3, page 733-737 ISSN 1755-098X 1755-0998 Genetics Ecology, Evolution, Behavior and Systematics Biotechnology journal-article 2009 crwiley https://doi.org/10.1111/j.1755-0998.2008.02475.x 2023-11-09T13:35:44Z Abstract Microsatellite markers for Atlantic cod ( Gadus morhua ) were identified from a collection of 30 630 expressed sequence tags. Primers were designed for 395 microsatellites and 155 were successfully amplified. Allele number varied from 1 to 26 (average 6.34). Average observed and expected heterozygosities were 0.50 and 0.54, respectively. A subset of 105 microsatellites tested for Mendelian segregation showed no significant distortion of segregation when correcting for multiple tests. Null alleles were detected at four loci. Significant blastx matches were found for 23 loci. These microsatellites will be used to create a linkage map to enhance genetic selection in commercial cod breeding. Article in Journal/Newspaper atlantic cod Gadus morhua Wiley Online Library (via Crossref) Molecular Ecology Resources 9 3 733 737 |
institution |
Open Polar |
collection |
Wiley Online Library (via Crossref) |
op_collection_id |
crwiley |
language |
English |
topic |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
spellingShingle |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology HIGGINS, B. HUBERT, S. SIMPSON, G. STONE, C. BOWMAN, S. Characterization of 155 EST‐derived microsatellites from Atlantic cod ( Gadus morhua ) and validation for linkage mapping |
topic_facet |
Genetics Ecology, Evolution, Behavior and Systematics Biotechnology |
description |
Abstract Microsatellite markers for Atlantic cod ( Gadus morhua ) were identified from a collection of 30 630 expressed sequence tags. Primers were designed for 395 microsatellites and 155 were successfully amplified. Allele number varied from 1 to 26 (average 6.34). Average observed and expected heterozygosities were 0.50 and 0.54, respectively. A subset of 105 microsatellites tested for Mendelian segregation showed no significant distortion of segregation when correcting for multiple tests. Null alleles were detected at four loci. Significant blastx matches were found for 23 loci. These microsatellites will be used to create a linkage map to enhance genetic selection in commercial cod breeding. |
format |
Article in Journal/Newspaper |
author |
HIGGINS, B. HUBERT, S. SIMPSON, G. STONE, C. BOWMAN, S. |
author_facet |
HIGGINS, B. HUBERT, S. SIMPSON, G. STONE, C. BOWMAN, S. |
author_sort |
HIGGINS, B. |
title |
Characterization of 155 EST‐derived microsatellites from Atlantic cod ( Gadus morhua ) and validation for linkage mapping |
title_short |
Characterization of 155 EST‐derived microsatellites from Atlantic cod ( Gadus morhua ) and validation for linkage mapping |
title_full |
Characterization of 155 EST‐derived microsatellites from Atlantic cod ( Gadus morhua ) and validation for linkage mapping |
title_fullStr |
Characterization of 155 EST‐derived microsatellites from Atlantic cod ( Gadus morhua ) and validation for linkage mapping |
title_full_unstemmed |
Characterization of 155 EST‐derived microsatellites from Atlantic cod ( Gadus morhua ) and validation for linkage mapping |
title_sort |
characterization of 155 est‐derived microsatellites from atlantic cod ( gadus morhua ) and validation for linkage mapping |
publisher |
Wiley |
publishDate |
2009 |
url |
http://dx.doi.org/10.1111/j.1755-0998.2008.02475.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2008.02475.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2008.02475.x |
genre |
atlantic cod Gadus morhua |
genre_facet |
atlantic cod Gadus morhua |
op_source |
Molecular Ecology Resources volume 9, issue 3, page 733-737 ISSN 1755-098X 1755-0998 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1111/j.1755-0998.2008.02475.x |
container_title |
Molecular Ecology Resources |
container_volume |
9 |
container_issue |
3 |
container_start_page |
733 |
op_container_end_page |
737 |
_version_ |
1784266023406403584 |