Characterization of 155 EST‐derived microsatellites from Atlantic cod ( Gadus morhua ) and validation for linkage mapping

Abstract Microsatellite markers for Atlantic cod ( Gadus morhua ) were identified from a collection of 30 630 expressed sequence tags. Primers were designed for 395 microsatellites and 155 were successfully amplified. Allele number varied from 1 to 26 (average 6.34). Average observed and expected he...

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Bibliographic Details
Published in:Molecular Ecology Resources
Main Authors: HIGGINS, B., HUBERT, S., SIMPSON, G., STONE, C., BOWMAN, S.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2009
Subjects:
Genetics
Ecology, Evolution, Behavior and Systematics
Biotechnology
atlantic cod
Gadus morhua
Online Access:http://dx.doi.org/10.1111/j.1755-0998.2008.02475.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2008.02475.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2008.02475.x
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Summary:Abstract Microsatellite markers for Atlantic cod ( Gadus morhua ) were identified from a collection of 30 630 expressed sequence tags. Primers were designed for 395 microsatellites and 155 were successfully amplified. Allele number varied from 1 to 26 (average 6.34). Average observed and expected heterozygosities were 0.50 and 0.54, respectively. A subset of 105 microsatellites tested for Mendelian segregation showed no significant distortion of segregation when correcting for multiple tests. Null alleles were detected at four loci. Significant blastx matches were found for 23 loci. These microsatellites will be used to create a linkage map to enhance genetic selection in commercial cod breeding.

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