Characterization of 18 new microsatellite loci in Atlantic cod ( Gadus morhua L.)

Abstract Eighteen new microsatellite loci consisting of 10 di‐, 5 tri‐, 2 tetra‐ and 1 heptanucleotide repeats are introduced for the Atlantic cod ( Gadus morhua L.). All loci were co‐amplified in two polymerase chain reactions (plus two previously published microsatellites) and all products were ty...

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Bibliographic Details
Published in:Molecular Ecology Resources
Main Authors: SKIRNISDOTTIR, SIGURLAUG, PAMPOULIE, CHRISTOPHE, HAUKSDOTTIR, SIGURBJORG, SCHULTE, INGA, OLAFSSON, KRISTINN, HREGGVIDSSON, GUDMUNDUR O., HJORLEIFSDOTTIR, SIGRIDUR
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2008
Subjects:
Genetics
Ecology, Evolution, Behavior and Systematics
Biotechnology
atlantic cod
Gadus morhua
Online Access:http://dx.doi.org/10.1111/j.1755-0998.2008.02327.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2008.02327.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2008.02327.x
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Summary:Abstract Eighteen new microsatellite loci consisting of 10 di‐, 5 tri‐, 2 tetra‐ and 1 heptanucleotide repeats are introduced for the Atlantic cod ( Gadus morhua L.). All loci were co‐amplified in two polymerase chain reactions (plus two previously published microsatellites) and all products were typed clearly. The number of alleles per locus ranged from six (PGmo130) to 45 (PGmo76) and the observed heterozygosity ranged from 0.356 (PGmo130) to 0.957 (PGmo95). All loci except one followed Hardy–Weinberg expectations. Genetic linkage disequilibrium analysis between all pairs of loci did not yield any significant values.

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