Characterization of microsatellite loci in the Hawaiian endemic shrub Schiedea adamantis (Caryophyllaceae) and amplification in related species and genera

Abstract Schiedea adamantis is a rare, perennial shrub endemic to the Hawaiian island of Oahu where it consists of a single population. Using a nonradioactive protocol, 12 microsatellite primers were developed that consisted of di‐, tri‐, penta‐ and hexanucleotide repeats. Using multiplexed reaction...

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Bibliographic Details
Published in:Molecular Ecology Resources
Main Authors: CULLEY, T. M., WELLER, S. G., SAKAI, A. K., PUTNAM, K. A.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2008
Subjects:
Genetics
Ecology, Evolution, Behavior and Systematics
Biotechnology
Honckenya peploides
Online Access:http://dx.doi.org/10.1111/j.1755-0998.2008.02161.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2008.02161.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2008.02161.x
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Summary:Abstract Schiedea adamantis is a rare, perennial shrub endemic to the Hawaiian island of Oahu where it consists of a single population. Using a nonradioactive protocol, 12 microsatellite primers were developed that consisted of di‐, tri‐, penta‐ and hexanucleotide repeats. Using multiplexed reactions, all but two primers exhibited polymorphism with an average of 3.67 alleles per primer. Most primers also amplified in 28 additional Schiedea species, revealing wide applicability across the genus; eight and nine primers also amplified in Honckenya peploides and Silene lanceolata , respectively, related genera in the Caryophyllaceae. This is the first known report of microsatellite primers developed in Schiedea .

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