Isolation and characterization of microsatellite loci in the deep‐sea marine fish, the roundnose grenadier ( Coryphaenoides rupestris )

Abstract We developed polymerase chain reaction primers for eight dinucleotide microsatellite loci in the marine deep sea fish, roundnose grenadier ( Coryphaenoides rupestris ). All markers were obtained from a partial genomic DNA library, and characterized in 90 unrelated individuals from one putat...

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Bibliographic Details
Published in:Molecular Ecology Resources
Main Authors: KNUTSEN, HALVOR, LE GOFF‐VITRY, MARIE, FIANI, DAVID, HOELZEL, A. RUS
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2008
Subjects:
Genetics
Ecology, Evolution, Behavior and Systematics
Biotechnology
Mid-Atlantic Ridge
atlantic cod
Gadus morhua
Online Access:http://dx.doi.org/10.1111/j.1755-0998.2008.02132.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1755-0998.2008.02132.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1755-0998.2008.02132.x
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Summary:Abstract We developed polymerase chain reaction primers for eight dinucleotide microsatellite loci in the marine deep sea fish, roundnose grenadier ( Coryphaenoides rupestris ). All markers were obtained from a partial genomic DNA library, and characterized in 90 unrelated individuals from one putative population sampled on the Mid‐Atlantic Ridge. The number of alleles ranged from two to 61 with an average of 21 per locus. The observed heterozygosity levels ranged from 0.301 to 0.987 with an average of 0.672. Several of the markers amplified multiple alleles from either the Atlantic cod ( Gadus morhua ) or the deep‐sea fish roughhead grenadier ( Macrourus berglax ).

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