ANALYSIS OF THE B10.D2‐ H‐2 d m l MUTANT WITH MONOCLONAL ANTIBODIES

SUMMARY We compared H‐2D products of B10.D2 and the mutant B10.D2‐ H‐2 dml spleen cells using two monoclonal antibodies designated D3.179 and S11.7. D3.179 reacts with public specificities and appears to define more than one product as shown by cytotoxicity and binding studies. Strong reactivity was...

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Bibliographic Details
Published in:International Journal of Immunogenetics
Main Authors: Tonkonogy, Susan L., Rinchik, E. M., Amos, D. B.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 1983
Subjects:
Genetics (clinical)
Genetics
Molecular Biology
General Medicine
Immunology
DML
Online Access:http://dx.doi.org/10.1111/j.1744-313x.1983.tb00794.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1744-313X.1983.tb00794.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1744-313X.1983.tb00794.x
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Summary:SUMMARY We compared H‐2D products of B10.D2 and the mutant B10.D2‐ H‐2 dml spleen cells using two monoclonal antibodies designated D3.179 and S11.7. D3.179 reacts with public specificities and appears to define more than one product as shown by cytotoxicity and binding studies. Strong reactivity was obtained in both binding and cytotoxicity and binding studies. Strong reactivity was obtained in both binding and cytotoxicity to B10.D2 and marginal cytotoxicity and low binding were observed with B10.D2‐ H‐2 dml cells. The second monoclonal antibody S11.7, in contrast, reacts only with H‐2D d bearing strains and gave strong binding to B10.D2‐ H‐2 dml and weaker binding to B10.D2. Taken together, the observations indicate that the mutant not only expresses H‐2D , encoded antigens but bear quantitatively greater amounts of some specificities than the parent. A Product of approximately 46,000 daltons was immunoprecipitated from B10.D2 cells by D3.179. Very small amounts of this product were seen in B10.D2‐ H‐2 dml immunoprecipitates.

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