Expression of carbonic anhydrases I/II and the correlation to clinical aspects of oral squamous cell carcinoma analyzed using tissue microarray

• Published in: Journal of Oral Pathology & Medicine
• Main Authors: Liu, Chia‐Ming, Lin, Yueh‐Min, Yeh, Kun‐Tu, Chen, Mu‐Kuan, Chang, Jer‐Hwa, Chen, Chih‐Jung, Chou, Ming‐Yung, Yang, Shun‐Fa, Chien, Ming‐Hsien
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2012
• Subjects: Carbonic acid
• Online Access: http://dx.doi.org/10.1111/j.1600-0714.2012.01135.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0714.2012.01135.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0714.2012.01135.x

J Oral Pathol Med (2012) 41 : 533–539 Background: Carbonic anhydrases (CA), a family of metalloenzymes, play an important role in catalyzing the equilibration of carbon dioxide (CO 2 ) and carbonic acid (H 2 CO 3 ). The role of CAs in tumorigenesis is controversial, especially regarding the expression of CA isoenzymes between various tumor types. This study explores the correlation between the expressions of CA I and CA II and the characteristic features of oral cancer. Methods: We immunohistochemically examined the expressions of CA I and CA II in 279 cases of oral squamous cell carcinoma (OSCC) using tissue microarrays. Additionally, the oral cancer cell line SCC‐9 was used to confirm the relationship between CA I and CA II expression and cell growth. Results: We found a significant correlation between positive CA I and CA II stains and OSCC for more advanced clinical stage ( P = 0.014 or 0.012) and larger tumor size ( P = 0.008 or 0.038), but not for positive lymph node metastasis, distal metastasis, and recurrence. In vitro analysis also showed that treatment with a CA inhibitor, acetazolamide, inhibited the growth of SCC‐9 cells. Conclusion: We conclude that expressions of CA I and CA II in OSCC samples can be used to predict local tumor growth in OSCC patients.