PERMANENT GENETIC RESOURCES: PCR primers for 100 microsatellites in red drum ( Sciaenops ocellatus)

• Published in: Molecular Ecology Resources
• Main Authors: KARLSSON, STEN, RENSHAW, MARK A., REXROAD III, CAIRD E., GOLD, JOHN R.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2008
• Subjects: Red drum; Sciaenops ocellatus
• Online Access: http://dx.doi.org/10.1111/j.1471-8286.2007.01969.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2007.01969.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2007.01969.x

Abstract One hundred nuclear‐encoded microsatellites from a genomic library of red drum ( Sciaenops ocellatus ) were isolated and characterized. Eight microsatellites had tetranucleotide motifs; 92 had dinucleotide motifs. The average number of alleles per microsatellite (sample of 22–24 fish) was 17.7 (range = 2–30); gene diversity averaged 0.796 (range = 0.227–1.000). Following Bonferroni correction, genotype frequencies at 90 microsatellites did not deviate significantly from Hardy–Weinberg equilibrium expectations. Occurrence of null alleles was inferred at 15 microsatellites; alleles differing by only a single base were observed at 11 microsatellites. The microsatellites developed should prove useful for population‐genetic studies of ‘wild’ red drum and in construction of a genetic map.