PERMANENT GENETIC RESOURCES: PCR primers for 100 microsatellites in red drum ( Sciaenops ocellatus)
Abstract One hundred nuclear‐encoded microsatellites from a genomic library of red drum ( Sciaenops ocellatus ) were isolated and characterized. Eight microsatellites had tetranucleotide motifs; 92 had dinucleotide motifs. The average number of alleles per microsatellite (sample of 22–24 fish) was 1...
Published in: | Molecular Ecology Resources |
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Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2008
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Subjects: | |
Online Access: | http://dx.doi.org/10.1111/j.1471-8286.2007.01969.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2007.01969.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2007.01969.x |
Summary: | Abstract One hundred nuclear‐encoded microsatellites from a genomic library of red drum ( Sciaenops ocellatus ) were isolated and characterized. Eight microsatellites had tetranucleotide motifs; 92 had dinucleotide motifs. The average number of alleles per microsatellite (sample of 22–24 fish) was 17.7 (range = 2–30); gene diversity averaged 0.796 (range = 0.227–1.000). Following Bonferroni correction, genotype frequencies at 90 microsatellites did not deviate significantly from Hardy–Weinberg equilibrium expectations. Occurrence of null alleles was inferred at 15 microsatellites; alleles differing by only a single base were observed at 11 microsatellites. The microsatellites developed should prove useful for population‐genetic studies of ‘wild’ red drum and in construction of a genetic map. |
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