New microsatellite markers for the bush rat, Rattus fuscipes greyii: characterization and cross‐species amplification
Abstract We describe here 16 new microsatellite markers for the bush rat, Rattus fuscipes greyii , and characterize their cross‐species amplification within the Australian Rattus and at a greater level of divergence in Rattus rattus and Rattus norvegicus . Within R. f. greyii , all of the loci are h...
| Published in: | Molecular Ecology Notes |
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| Main Authors: | , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
2007
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1111/j.1471-8286.2007.01848.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2007.01848.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2007.01848.x |
| Summary: | Abstract We describe here 16 new microsatellite markers for the bush rat, Rattus fuscipes greyii , and characterize their cross‐species amplification within the Australian Rattus and at a greater level of divergence in Rattus rattus and Rattus norvegicus . Within R. f. greyii , all of the loci are highly polymorphic, with six to 24 alleles per locus across the species range and expected heterozygosity ranging from 0.48 to 0.90 per locus within a sample of 24 rats from a large population on Kangaroo Island. Cross‐species amplification rates were approximately 87% within the Australian Rattus and approximately 50% within R. rattus and R. norvegicus. These loci are highly polymorphic with a high success rate of cross‐species amplification, making them potentially useful for a wide range of genetic studies. |
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