Multiplex panels of polymorphic microsatellite loci for two cryptic bat species of the genus Pipistrellus, developed by cross‐species amplification within the family Vespertilionidae

• Published in: Molecular Ecology Notes
• Main Authors: KAŇUCH, P., FORNŮSKOVÁ, A., BARTONIČKA, T., BRYJA, J.
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2007
• Subjects: Pipistrellus pipistrellus
• Online Access: http://dx.doi.org/10.1111/j.1471-8286.2007.01733.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2007.01733.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2007.01733.x

Abstract The paper presents multiplex panels of polymorphic microsatellites for two closely related cryptic species Pipistrellus pipistrellus and Pipistrellus pygmaeus . We tested the cross‐species amplification of 34 microsatellite loci, originally developed for five vespertilionid bat species. Ten and nine polymorphic loci in P . pipistrellus (mean number of alleles per locus = 10.5) and P . pygmaeus (8.1), respectively, in three multiplex polymerase chain reactions per species were amplified. All loci can be analysed in a single fragment analysis and can be used as markers to the study of evolution and the ecology of structured populations of socially living bats.