Multiplex panels of polymorphic microsatellite loci for two cryptic bat species of the genus Pipistrellus, developed by cross‐species amplification within the family Vespertilionidae

Abstract The paper presents multiplex panels of polymorphic microsatellites for two closely related cryptic species Pipistrellus pipistrellus and Pipistrellus pygmaeus . We tested the cross‐species amplification of 34 microsatellite loci, originally developed for five vespertilionid bat species. Ten...

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Bibliographic Details
Published in:Molecular Ecology Notes
Main Authors: KAŇUCH, P., FORNŮSKOVÁ, A., BARTONIČKA, T., BRYJA, J.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2007
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Online Access:http://dx.doi.org/10.1111/j.1471-8286.2007.01733.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2007.01733.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2007.01733.x
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Summary:Abstract The paper presents multiplex panels of polymorphic microsatellites for two closely related cryptic species Pipistrellus pipistrellus and Pipistrellus pygmaeus . We tested the cross‐species amplification of 34 microsatellite loci, originally developed for five vespertilionid bat species. Ten and nine polymorphic loci in P . pipistrellus (mean number of alleles per locus = 10.5) and P . pygmaeus (8.1), respectively, in three multiplex polymerase chain reactions per species were amplified. All loci can be analysed in a single fragment analysis and can be used as markers to the study of evolution and the ecology of structured populations of socially living bats.