Nine new polymorphic microsatellite loci for the amplification of archived otolith DNA of Atlantic cod, Gadus morhua L.

Abstract Nine out of 22 microsatellite primers tested were successfully amplified on three samples of cod Gadus morhua L. (two contemporary and one archived otolith samples). All loci were polymorphic (5–23 alleles/locus). The average observed heterozygosity across loci and samples was 0.625, rangin...

Full description

Bibliographic Details
Published in:Molecular Ecology Notes
Main Authors: JAKOBSDÓTTIR, KLARA BJÖRG, JÖRUNDSDÓTTIR, þÓRA DÖGG, SKÍRNISDÓTTIR, SIGURLAUG, HJÖRLEIFSDÓTTIR, SIGRÍÐUR, HREGGVIÐSSON, GUÐMUNDUR Ó., DANÍELSDÓTTIR, ANNA KRISTÍN, PAMPOULIE, CHRISTOPHE
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2006
Subjects:
atlantic cod
Gadus morhua
Online Access:http://dx.doi.org/10.1111/j.1471-8286.2005.01223.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2005.01223.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2005.01223.x
Description
Summary:Abstract Nine out of 22 microsatellite primers tested were successfully amplified on three samples of cod Gadus morhua L. (two contemporary and one archived otolith samples). All loci were polymorphic (5–23 alleles/locus). The average observed heterozygosity across loci and samples was 0.625, ranging from 0.294 to 0.895 at each locus. All loci were under Hardy–Weinberg equilibrium, except PGmo56 that showed significant excess of heterozygotes in all studied samples. The isolated loci were suitable for degraded DNA and therefore useful for conducting a long‐term temporal study with DNA obtained from archived otoliths of cod.

Similar Items