Isolation and characterization of microsatellite loci in the icefish Chionodraco rastrospinosus (Perciformes, Notothenioidea, Channichthyidae)

Abstract We characterized eight polymorphic microsatellites in the icefish Chionodraco rastrospinosus (Perciformes, Notothenioidea, Channichthyidae). Microsatellites were isolated from a partial genomic library enriched for an AC motif. Chionodraco rastrospinosus is an endemic species inhabiting sou...

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Bibliographic Details
Published in:Molecular Ecology Notes
Main Authors: PAPETTI, CHIARA, ZANE, LORENZO, PATARNELLO, TOMASO
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2005
Subjects:
Antarctic
Antarctic Peninsula
South Orkney Islands
South Shetland Islands
Southern Ocean
The Antarctic
Antarc*
Icefish
Online Access:http://dx.doi.org/10.1111/j.1471-8286.2005.01194.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2005.01194.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2005.01194.x
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Summary:Abstract We characterized eight polymorphic microsatellites in the icefish Chionodraco rastrospinosus (Perciformes, Notothenioidea, Channichthyidae). Microsatellites were isolated from a partial genomic library enriched for an AC motif. Chionodraco rastrospinosus is an endemic species inhabiting southern ocean waters surrounding the Antarctic Peninsula, the South Shetland Islands, and the South Orkney Islands. An excess of homozygotes was observed in seven out of the eight investigated loci; however, presence of null alleles was detected only for three of them suggesting that other factors may act in reducing heterozygosity. These molecular markers will be useful to investigate icefish genetic structure, possibly providing insights on its effective population size and demographic history.

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