Genotyping of pantophysin I ( Pan I) of Atlantic cod ( Gadus morhua L.) by allele‐specific PCR
Abstract The two main allelic variants of the Atlantic cod ( Gadus morhua L.) pantophysin I ( Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. W...
| Published in: | Molecular Ecology Notes |
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| Main Authors: | , , , |
| Format: | Article in Journal/Newspaper |
| Language: | English |
| Published: |
Wiley
2005
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| Subjects: | |
| Online Access: | http://dx.doi.org/10.1111/j.1471-8286.2005.01178.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2005.01178.x https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2005.01178.x |
| Summary: | Abstract The two main allelic variants of the Atlantic cod ( Gadus morhua L.) pantophysin I ( Pan I) locus have different frequencies within different cod stocks. The Dra I polymorphism which distinguishes the two alleles can thus be used for discrimination of coastal and offshore cod populations. We present a new method for Pan I genotyping using fluorescent allele‐specific duplex polymerase chain reaction (PCR). This method is more rapid, reliable and cost‐effective than the previously published method and it is not affected by DNA source and quality. This improvement is important for studies demanding high throughput and accuracy of Pan I genotyping |
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