Isolation and cross‐familial amplification of 41 microsatellites for the brook charr ( Salvelinus fontinalis)

• Published in: Molecular Ecology Notes
• Main Authors: PERRY, GUY M. L., KING, TIM L., ST.‐CYR, JÉRÔME, VALCOURT, MANON, BERNATCHEZ, LOUIS
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2005
• Subjects: Arctic; Arctic charr; Atlantic salmon; Salmo salar; Salvelinus alpinus
• Online Access: http://dx.doi.org/10.1111/j.1471-8286.2005.00922.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2005.00922.x; https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2005.00922.x

Abstract The brook charr ( Salvelinus fontinalis Osteichthyes: Salmonidae) is a phenotypically diverse fish species inhabiting much of North America. But relatively few genetic diagnostic resources are available for this fish species. We isolated 41 microsatellites from S. fontinalis polymorphic in one or more species of salmonid fish. Thirty‐seven were polymorphic in brook charr, 15 in the congener Arctic charr ( Salvelinus alpinus ) and 14 in the lake charr ( Salvelinus namaycush ). Polymorphism was also relatively high in Oncorhynchus , where 21 loci were polymorphic in rainbow trout ( Oncorhynchus mykiss ) and 16 in cutthroat trout ( Oncorhynchus clarkii ) but only seven and four microsatellite loci were polymorphic in the more distantly related lake whitefish ( Coregonus clupeaformis ) and Atlantic salmon ( Salmo salar ), respectively. One duplicated locus ( Sfo228Lav ) was polymorphic at both duplicates in S. fontinalis .