Isolation and cross‐familial amplification of 41 microsatellites for the brook charr ( Salvelinus fontinalis)

Abstract The brook charr ( Salvelinus fontinalis Osteichthyes: Salmonidae) is a phenotypically diverse fish species inhabiting much of North America. But relatively few genetic diagnostic resources are available for this fish species. We isolated 41 microsatellites from S. fontinalis polymorphic in...

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Bibliographic Details
Published in:Molecular Ecology Notes
Main Authors: PERRY, GUY M. L., KING, TIM L., ST.‐CYR, JÉRÔME, VALCOURT, MANON, BERNATCHEZ, LOUIS
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2005
Subjects:
Arctic
Arctic charr
Atlantic salmon
Salmo salar
Salvelinus alpinus
Online Access:http://dx.doi.org/10.1111/j.1471-8286.2005.00922.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1471-8286.2005.00922.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1471-8286.2005.00922.x
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Summary:Abstract The brook charr ( Salvelinus fontinalis Osteichthyes: Salmonidae) is a phenotypically diverse fish species inhabiting much of North America. But relatively few genetic diagnostic resources are available for this fish species. We isolated 41 microsatellites from S. fontinalis polymorphic in one or more species of salmonid fish. Thirty‐seven were polymorphic in brook charr, 15 in the congener Arctic charr ( Salvelinus alpinus ) and 14 in the lake charr ( Salvelinus namaycush ). Polymorphism was also relatively high in Oncorhynchus , where 21 loci were polymorphic in rainbow trout ( Oncorhynchus mykiss ) and 16 in cutthroat trout ( Oncorhynchus clarkii ) but only seven and four microsatellite loci were polymorphic in the more distantly related lake whitefish ( Coregonus clupeaformis ) and Atlantic salmon ( Salmo salar ), respectively. One duplicated locus ( Sfo228Lav ) was polymorphic at both duplicates in S. fontinalis .

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