Rapid development of polyclonal antisera against infectious salmon anaemia virus and its optimization and application as a diagnostic tool

Abstract Infectious salmon anaemia is an important disease of Atlantic salmon. One of the current methods of diagnosis is the indirect fluorescent antibody test (IFAT), using a monoclonal antibody specific to the haemagglutinin of the virus. The conformationally dependent nature of this antibody cou...

Full description

Bibliographic Details
Published in:Journal of Fish Diseases
Main Authors: McBeath, S J, Ellis, L M, Cook, P F, Wilson, L, Urquhart, K L, Bricknell, I R
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2006
Subjects:
Atlantic salmon
Online Access:http://dx.doi.org/10.1111/j.1365-2761.2006.00720.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2761.2006.00720.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1365-2761.2006.00720.x
Description
Summary:Abstract Infectious salmon anaemia is an important disease of Atlantic salmon. One of the current methods of diagnosis is the indirect fluorescent antibody test (IFAT), using a monoclonal antibody specific to the haemagglutinin of the virus. The conformationally dependent nature of this antibody could be a drawback in its usefulness in other tests. This study describes the development and optimization of a polyclonal antiserum against infectious salmon anaemia virus, including a method of separating virus from cell culture components within culture supernatant. The antiserum was subsequently optimized for use in a variety of immunological diagnostic tests, including IFAT and an alkaline phosphatase‐based immunoassay, and Western blot.

Similar Items