Suppression of Thermal Denaturation of Myosin Subfragment‐1 of Alaska Pollack ( Theragra chalcogramma) by Sorbitol and Accelerated Inactivation by Pyrophosphate

• Published in: Journal of Food Science
• Main Author: KONNO, KUNIHIKO
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 1992
• Subjects: Theragra chalcogramma; Alaska
• Online Access: http://dx.doi.org/10.1111/j.1365-2621.1992.tb05471.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2621.1992.tb05471.x; http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2621.1992.tb05471.x/fullpdf

ABSTRACT Thermal denaturation of myosin subfragment‐1 (S‐1), prepared from myofibrils (Mf) of Alaska pollack dorsal muscle by chymotryptic digestion, was well suppressed by sorbitol addition in a concentration dependent relationship. ATP and ADP also suppressed the ATPase inactivation significantly at different degrees. On the contrary, pyrophosphate (PPi) accelerated the inactivation by several‐fold; half the maximal acceleration occurred at about 30 μn, M PPi concentration. The accelerated inactivation by PPi addition was remarkable at high‐salt medium such as 0.5M KC1.