Suppression of Thermal Denaturation of Myosin Subfragment‐1 of Alaska Pollack ( Theragra chalcogramma) by Sorbitol and Accelerated Inactivation by Pyrophosphate
ABSTRACT Thermal denaturation of myosin subfragment‐1 (S‐1), prepared from myofibrils (Mf) of Alaska pollack dorsal muscle by chymotryptic digestion, was well suppressed by sorbitol addition in a concentration dependent relationship. ATP and ADP also suppressed the ATPase inactivation significantly...
Published in: | Journal of Food Science |
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Main Author: | |
Format: | Article in Journal/Newspaper |
Language: | English |
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Wiley
1992
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Online Access: | http://dx.doi.org/10.1111/j.1365-2621.1992.tb05471.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1365-2621.1992.tb05471.x http://onlinelibrary.wiley.com/wol1/doi/10.1111/j.1365-2621.1992.tb05471.x/fullpdf |
Summary: | ABSTRACT Thermal denaturation of myosin subfragment‐1 (S‐1), prepared from myofibrils (Mf) of Alaska pollack dorsal muscle by chymotryptic digestion, was well suppressed by sorbitol addition in a concentration dependent relationship. ATP and ADP also suppressed the ATPase inactivation significantly at different degrees. On the contrary, pyrophosphate (PPi) accelerated the inactivation by several‐fold; half the maximal acceleration occurred at about 30 μn, M PPi concentration. The accelerated inactivation by PPi addition was remarkable at high‐salt medium such as 0.5M KC1. |
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