Improved primer sequences for the mitochondrial ND1, ND3/4 and ND5/6 segments in salmonid fishes: application to RFLP analysis of Atlantic salmon

New specific primers for the mtDNA segments ND1, ND3/4 and ND5/6 designed from the rainbow trout sequence, improved PCR amplification for salmonid fishes. RFLP analysis revealed restriction site variation for all three segments in Atlantic salmon. Eleven haplotypes were detected in a screening of 30...

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Bibliographic Details
Published in:Journal of Fish Biology
Main Authors: Nielsen, E. E., Hansen, M. M., Mensberg, K.‐L. D.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 1998
Subjects:
Atlantic salmon
Online Access:http://dx.doi.org/10.1111/j.1095-8649.1998.tb00122.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1998.tb00122.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1998.tb00122.x
Description
Summary:New specific primers for the mtDNA segments ND1, ND3/4 and ND5/6 designed from the rainbow trout sequence, improved PCR amplification for salmonid fishes. RFLP analysis revealed restriction site variation for all three segments in Atlantic salmon. Eleven haplotypes were detected in a screening of 30 individuals from four European populations.

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