Measurements on ground or sectioned otoliths: possibilities of bias

Back‐calculation usually requires measurements of growth marks revealed on otoliths by specific preparations. The standardization of the grinding (or sectioning) plane is necessary, but difficult, especially along the antero‐posterior axis. In order to show the importance of the grinding plane, tetr...

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Bibliographic Details
Published in:Journal of Fish Biology
Main Authors: Panfili, J., Ximenes, M.‐C.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 1992
Subjects:
Online Access:http://dx.doi.org/10.1111/j.1095-8649.1992.tb02650.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1095-8649.1992.tb02650.x
https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1095-8649.1992.tb02650.x
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Summary:Back‐calculation usually requires measurements of growth marks revealed on otoliths by specific preparations. The standardization of the grinding (or sectioning) plane is necessary, but difficult, especially along the antero‐posterior axis. In order to show the importance of the grinding plane, tetracycline labelling of eel otoliths ( Anguilla anguilla L.) has been used. This marking has a calcio‐traumatic effect on otoliths, which can be revealed with staining techniques. Unless the grinding plane is incorrect, the tetracycline labelling and the staining of the ground surface are then superposed.