Low hydrogen peroxide production in mitochondria of the long‐lived Arctica islandica: underlying mechanisms for slow aging

• Published in: Aging Cell
• Main Authors: Munro, Daniel, Pichaud, Nicolas, Paquin, Frédérique, Kemeid, Vincent, Blier, Pierre U.
• Other Authors: NSERC, FONCER, Réseau Aquaculture Québec, NSERC Discovery
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2013
• Subjects: Arctica islandica
• Online Access: http://dx.doi.org/10.1111/acel.12082; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Facel.12082; https://onlinelibrary.wiley.com/doi/pdf/10.1111/acel.12082; https://onlinelibrary.wiley.com/doi/full-xml/10.1111/acel.12082

Summary The observation of an inverse relationship between lifespan and mitochondrial H 2 O 2 production rate would represent strong evidence for the disputed oxidative stress theory of aging. Studies on this subject using invertebrates are surprisingly lacking, despite their significance in both taxonomic richness and biomass. Bivalve mollusks represent an interesting taxonomic group to challenge this relationship. They are exposed to environmental constraints such as microbial H 2 S , anoxia/reoxygenation, and temperature variations known to elicit oxidative stress. Their mitochondrial electron transport system is also connected to an alternative oxidase that might improve their ability to modulate reactive oxygen species ( ROS ) yield. Here, we compared H 2 O 2 production rates in isolated mantle mitochondria between the longest‐living metazoan—the bivalve A rctica islandica —and two taxonomically related species of comparable size. In an attempt to test mechanisms previously proposed to account for a reduction of ROS production in long‐lived species, we compared oxygen consumption of isolated mitochondria and enzymatic activity of different complexes of the electron transport system in the two species with the greatest difference in longevity. We found that A . islandica mitochondria produced significantly less H 2 O 2 than those of the two short‐lived species in nearly all conditions of mitochondrial respiration tested, including forward, reverse, and convergent electron flow. Alternative oxidase activity does not seem to explain these differences. However, our data suggest that reduced complex I and III activity can contribute to the lower ROS production of A . islandica mitochondria, in accordance with previous studies. We further propose that a lower complex II activity could also be involved.