Pyrosequencing andde novoassembly of Antarctic krill ( Euphausia superba)transcriptome to study the adaptability of krill to climate‐induced environmental changes

• Published in: Molecular Ecology Resources
• Main Authors: Meyer, B., Martini, P., Biscontin, A., De Pittà, C., Romualdi, C., Teschke, M., Frickenhaus, S., Harms, L., Freier, U., Jarman, S., Kawaguchi, S.
• Other Authors: Ministry of Education and Science
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2015
• Subjects: Antarctic; Antarc*; Antarctic Krill; Euphausia superba
• Online Access: http://dx.doi.org/10.1111/1755-0998.12408; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2F1755-0998.12408; https://onlinelibrary.wiley.com/doi/pdf/10.1111/1755-0998.12408; https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1755-0998.12408

Abstract The A ntarctic krill, E uphausia superba , has a key position in the S outhern O cean food web by serving as direct link between primary producers and apex predators. The south‐west A tlantic sector of the S outhern O cean, where the majority of the krill population is located, is experiencing one of the most profound environmental changes worldwide. Up to now, we have only cursory information about krill's genomic plasticity to cope with the ongoing environmental changes induced by anthropogenic CO 2 emission. The genome of krill is not yet available due to its large size (about 48 G bp). Here, we present two c DNA normalized libraries from whole krill and krill heads sampled in different seasons that were combined with two data sets of krill transcriptome projects, already published, to produce the first knowledgebase krill ‘master’ transcriptome. The new library produced 25% more E . superba transcripts and now includes nearly all the enzymes involved in the primary oxidative metabolism ( G lycolysis, K rebs cycle and oxidative phosphorylation) as well as all genes involved in glycogenesis, glycogen breakdown, gluconeogenesis, fatty acid synthesis and fatty acids β ‐oxidation. With these features, the ‘master’ transcriptome provides the most complete picture of metabolic pathways in A ntarctic krill and will provide a major resource for future physiological and molecular studies. This will be particularly valuable for characterizing the molecular networks that respond to stressors caused by the anthropogenic CO 2 emissions and krill's capacity to cope with the ongoing environmental changes in the A tlantic sector of the S outhern O cean.