Pyocin‐mediated antagonistic interactions in Pseudomonas spp. isolated in James Ross Island, Antarctica

Summary Interactions within bacterial communities are frequently mediated by the production of antimicrobial agents. Despite the increasing interest in research of new antimicrobials, studies describing antagonistic interactions among cold‐adapted microorganisms are still rare. Our study assessed th...

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Bibliographic Details
Published in:Environmental Microbiology
Main Authors: Snopková, Kateřina, Dufková, Kristýna, Chamrád, Ivo, Lenobel, René, Čejková, Darina, Kosina, Marcel, Hrala, Matěj, Holá, Veronika, Sedláček, Ivo, Šmajs, David
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2021
Subjects:
Antarctic
Ross Island
Antarc*
Antarctica
James Ross Island
Online Access:http://dx.doi.org/10.1111/1462-2920.15809
https://onlinelibrary.wiley.com/doi/pdf/10.1111/1462-2920.15809
https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1462-2920.15809
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Summary:Summary Interactions within bacterial communities are frequently mediated by the production of antimicrobial agents. Despite the increasing interest in research of new antimicrobials, studies describing antagonistic interactions among cold‐adapted microorganisms are still rare. Our study assessed the antimicrobial interactions of 36 Antarctic Pseudomonas spp. and described the genetic background of these interactions in selected strains. The overall bacteriocinogeny was greater compared to mesophilic Pseudomonas non‐aeruginosa species. R‐type tailocins were detected on transmission electron micrographs in 16 strains (44.4%); phylogenetic analysis of the corresponding gene clusters revealed that the P . prosekii CCM 8878 tailocin was related to the Rp3 group, whereas the tailocin in Pseudomonas sp. CCM 8880 to the Rp4 group. Soluble antimicrobials were produced by eight strains (22.‐2%); gene mining found pyocin L homologues in the genomes of P . prosekii CCM 8881 and CCM 8879 and pyocin S9‐like homologues in P . prosekii CCM 8881 and Pseudomonas sp. CCM 8880. Analysis of secretomes confirmed the production of all S‐ and L‐type pyocin genes. Our results suggest that bacteriocin‐based inhibition plays an important role in interactions among Antarctic soil bacteria, and these native, cold‐adapted microorganisms could be a promising source of new antimicrobials.

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