Changes in stable nitrogen isotopes of plants, bulk soil and soil dissolved N during ecosystem retrogression in boreal forest

• Published in: Ecological Research
• Main Authors: Hyodo, Fujio, Takebayashi, Yu, Makabe, Akiko, Wardle, David A., Koba, Keisuke
• Other Authors: Research Institute for Humanity and Nature
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2021
• Subjects: Northern Sweden
• Online Access: http://dx.doi.org/10.1111/1440-1703.12208; https://onlinelibrary.wiley.com/doi/pdf/10.1111/1440-1703.12208; https://onlinelibrary.wiley.com/doi/full-xml/10.1111/1440-1703.12208

Abstract Stable nitrogen isotope ratios (δ 15 N) of plants and soil have been used to study changes in the N cycle during ecosystem succession and retrogression. However, little is known about how δ 15 N of soil mineral N and dissolved organic N (DON) change during retrogression, despite their potential to inform on processes contributing to N loss. Here, we examined the δ 15 N of NH 4 + and DON together with δ 15 N of the dominant plant species and bulk soil across a 5,000‐year‐old retrogressive chronosequence of forested islands in northern Sweden. The δ 15 N of bulk soil N, NH 4 + and DON did not change greatly during retrogression, suggesting that there are no major losses of N from the system. The δ 15 N of NH 4 + and DON was significantly correlated with that of bulk soil N across islands, indicating that bulk soil N is an important determinant of the δ 15 N of dissolved soil N. The δ 15 N of DON was significantly higher than those of NH 4 + and bulk soil N, probably because of the inclusion of microbial N to the DON fraction. Despite the lack of changes in δ 15 N of soil N as retrogression proceeded, the δ 15 N of most plant species increased. These results suggest that despite the relative importance of the three underlying mechanisms involved is unclear, the N resources of plants change in response to retrogression: they have an increasing reliance on DON, a decreasing dependence on N transferred from the mycorrhizal fungi and reduced reliance on N from surface soil layers as retrogression proceeds.