A set of polymorphic SSR loci for subarctic willow ( Salix lanata, S. lapponum and S. herbacea)

Abstract We report the development of seven microsatellite markers from a library enriched with [CA] 15 , [GA] 15 , [AAG] 8 and [ATG] 8 . The library was constructed from Icelandic Salix lanata leaf DNA, and seven highly polymorphic single locus products were tested on a range of Scottish and Icelan...

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Bibliographic Details
Published in:Molecular Ecology Notes
Main Authors: Stamati, K., Blackie, S., Brown, J. W. S., Russell, J.
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 2003
Subjects:
Salix herbacea
Salix lanata
Subarctic
Online Access:http://dx.doi.org/10.1046/j.1471-8286.2003.00426.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-8286.2003.00426.x
https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-8286.2003.00426.x
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Summary:Abstract We report the development of seven microsatellite markers from a library enriched with [CA] 15 , [GA] 15 , [AAG] 8 and [ATG] 8 . The library was constructed from Icelandic Salix lanata leaf DNA, and seven highly polymorphic single locus products were tested on a range of Scottish and Icelandic S. lanata samples. These seven loci also amplify polymorphic products when tested for cross amplification in a range of Scottish and Icelandic samples of two other subarctic willows ( Salix lapponum and Salix herbacea ). The microsatellites developed in this study will provide the framework for comparisons with other ecosystem components, and will ultimately inform conservation strategies.

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