Species‐specific TaqMan probes for simultaneous identification of ( Gadus morhua L.), haddock ( Melanogrammus aeglefinus L.) and whiting ( Merlangius merlangus L.).

• Published in: Molecular Ecology Notes
• Main Authors: Taylor, Martin I., Fox, Clive, Rico, Ines, Rico, Ciro
• Format: Article in Journal/Newspaper
• Language: English
• Published: Wiley 2002
• Subjects: General Biochemistry, Genetics and Molecular Biology; Ecology; Biochemistry; Gadus morhua
• Online Access: http://dx.doi.org/10.1046/j.1471-8286.2002.00269.x; https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-8286.2002.00269.x; https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-8286.2002.00269.x

Abstract We report the development of a multiplex, single tube (TaqMan) PCR assay for the identification of three commercially important gadoid species, the cod ( Gadus morhua L.), the haddock ( Melanogrammus aeglefinus L.) and the whiting ( Merlangius merlangus L.). The assay unambiguously identifies known adult tissue samples, and ethanol‐preserved eggs from all three species with greater than 98% accuracy, providing a powerful tool for the development of catch independent stock assessment methods, mapping of spawning sites and the detection of commercial fraud in the fishing and food production industries.