Species‐specific TaqMan probes for simultaneous identification of ( Gadus morhua L.), haddock ( Melanogrammus aeglefinus L.) and whiting ( Merlangius merlangus L.).
Abstract We report the development of a multiplex, single tube (TaqMan) PCR assay for the identification of three commercially important gadoid species, the cod ( Gadus morhua L.), the haddock ( Melanogrammus aeglefinus L.) and the whiting ( Merlangius merlangus L.). The assay unambiguously identifi...
Published in: | Molecular Ecology Notes |
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Main Authors: | , , , |
Format: | Article in Journal/Newspaper |
Language: | English |
Published: |
Wiley
2002
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Subjects: | |
Online Access: | http://dx.doi.org/10.1046/j.1471-8286.2002.00269.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1471-8286.2002.00269.x https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1471-8286.2002.00269.x |
Summary: | Abstract We report the development of a multiplex, single tube (TaqMan) PCR assay for the identification of three commercially important gadoid species, the cod ( Gadus morhua L.), the haddock ( Melanogrammus aeglefinus L.) and the whiting ( Merlangius merlangus L.). The assay unambiguously identifies known adult tissue samples, and ethanol‐preserved eggs from all three species with greater than 98% accuracy, providing a powerful tool for the development of catch independent stock assessment methods, mapping of spawning sites and the detection of commercial fraud in the fishing and food production industries. |
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