Cloning, overexpression and characterization of micro‐myoglobin, a minimal heme‐binding fragment
We report the cloning and expression of micro‐myoglobin, a 78‐amino‐acid fragment containing residues 29–105 of sperm whale myoglobin, and spanning the region from mid‐helix B to mid‐helix G of the globin fold. In contrast to full‐length myoglobin and to mini‐myoglobin (residues 32–129), the micro‐m...
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crwiley:10.1046/j.1432-1327.2000.01114.x 2024-06-02T08:14:52+00:00 Cloning, overexpression and characterization of micro‐myoglobin, a minimal heme‐binding fragment Grandori, Rita Schwarzinger, Stephan Müller, Norbert 2000 http://dx.doi.org/10.1046/j.1432-1327.2000.01114.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1327.2000.01114.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1327.2000.01114.x en eng Wiley http://onlinelibrary.wiley.com/termsAndConditions#vor European Journal of Biochemistry volume 267, issue 4, page 1168-1172 ISSN 0014-2956 1432-1033 journal-article 2000 crwiley https://doi.org/10.1046/j.1432-1327.2000.01114.x 2024-05-03T11:13:06Z We report the cloning and expression of micro‐myoglobin, a 78‐amino‐acid fragment containing residues 29–105 of sperm whale myoglobin, and spanning the region from mid‐helix B to mid‐helix G of the globin fold. In contrast to full‐length myoglobin and to mini‐myoglobin (residues 32–129), the micro‐myoglobin apoprotein is almost unfolded. However, circular dichroism and absorption spectroscopy data indicate that this fragment is capable of folding into a functional heme‐binding unit forming a complex with the prosthetic group with characteristics similar to native myoglobin. Therefore, this case represents a new example of cofactor‐assisted folding. The experimental data suggest independence between myoglobin subdomains. Article in Journal/Newspaper Sperm whale Wiley Online Library European Journal of Biochemistry 267 4 1168 1172 |
institution |
Open Polar |
collection |
Wiley Online Library |
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crwiley |
language |
English |
description |
We report the cloning and expression of micro‐myoglobin, a 78‐amino‐acid fragment containing residues 29–105 of sperm whale myoglobin, and spanning the region from mid‐helix B to mid‐helix G of the globin fold. In contrast to full‐length myoglobin and to mini‐myoglobin (residues 32–129), the micro‐myoglobin apoprotein is almost unfolded. However, circular dichroism and absorption spectroscopy data indicate that this fragment is capable of folding into a functional heme‐binding unit forming a complex with the prosthetic group with characteristics similar to native myoglobin. Therefore, this case represents a new example of cofactor‐assisted folding. The experimental data suggest independence between myoglobin subdomains. |
format |
Article in Journal/Newspaper |
author |
Grandori, Rita Schwarzinger, Stephan Müller, Norbert |
spellingShingle |
Grandori, Rita Schwarzinger, Stephan Müller, Norbert Cloning, overexpression and characterization of micro‐myoglobin, a minimal heme‐binding fragment |
author_facet |
Grandori, Rita Schwarzinger, Stephan Müller, Norbert |
author_sort |
Grandori, Rita |
title |
Cloning, overexpression and characterization of micro‐myoglobin, a minimal heme‐binding fragment |
title_short |
Cloning, overexpression and characterization of micro‐myoglobin, a minimal heme‐binding fragment |
title_full |
Cloning, overexpression and characterization of micro‐myoglobin, a minimal heme‐binding fragment |
title_fullStr |
Cloning, overexpression and characterization of micro‐myoglobin, a minimal heme‐binding fragment |
title_full_unstemmed |
Cloning, overexpression and characterization of micro‐myoglobin, a minimal heme‐binding fragment |
title_sort |
cloning, overexpression and characterization of micro‐myoglobin, a minimal heme‐binding fragment |
publisher |
Wiley |
publishDate |
2000 |
url |
http://dx.doi.org/10.1046/j.1432-1327.2000.01114.x https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1327.2000.01114.x https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1327.2000.01114.x |
genre |
Sperm whale |
genre_facet |
Sperm whale |
op_source |
European Journal of Biochemistry volume 267, issue 4, page 1168-1172 ISSN 0014-2956 1432-1033 |
op_rights |
http://onlinelibrary.wiley.com/termsAndConditions#vor |
op_doi |
https://doi.org/10.1046/j.1432-1327.2000.01114.x |
container_title |
European Journal of Biochemistry |
container_volume |
267 |
container_issue |
4 |
container_start_page |
1168 |
op_container_end_page |
1172 |
_version_ |
1800738879957893120 |