Atlantic salmon ( Salmo salar L.) skin contains a novel kininogen and another cysteine proteinase inhibitor

We describe the purification and characterization of two novel cysteine proteinase inhibitors found in Atlantic salmon skin. One of these, salmon kininogen, has a molecular mass of 52 kDa as determined by matrix‐assisted laser desorption/ionization time‐of‐flight MS, is multiply charged with pI valu...

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Bibliographic Details
Published in:European Journal of Biochemistry
Main Authors: Ylönen, Anne, Rinne, Ari, Herttuainen, Jukka, Bøgwald, Jarl, Järvinen, Mikko, Kalkkinen, Nisse
Format: Article in Journal/Newspaper
Language:English
Published: Wiley 1999
Subjects:
Atlantic salmon
Salmo salar
Online Access:http://dx.doi.org/10.1046/j.1432-1327.1999.00950.x
https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1432-1327.1999.00950.x
https://febs.onlinelibrary.wiley.com/doi/pdf/10.1046/j.1432-1327.1999.00950.x
Description
Summary:We describe the purification and characterization of two novel cysteine proteinase inhibitors found in Atlantic salmon skin. One of these, salmon kininogen, has a molecular mass of 52 kDa as determined by matrix‐assisted laser desorption/ionization time‐of‐flight MS, is multiply charged with pI values of 4.0, 4.2 and 4.6 and shows homology to kininogens including the bradykinin motif. The other, salarin, has a molecular weight of 43 kDa, a pI of 5.1 and shows weak homology to cysteine proteinases. Both proteins are N‐ and O‐glycosylated and inhibit papain and ficin but not trypsin.

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